Osteogenesis effects of strontium-substituted hydroxyapatite coatings on true bone ceramic surfaces in vitro and in vivo
Osteogenesis effects of strontium-substituted hydroxyapatite coatings on true bone ceramic surfaces in vitro and in vivo
复制标题
锶取代羟基磷灰石涂层对真骨陶瓷表面的体外和体内成骨作用
DOI：
10.1088/1748-605x/aa89af
复制
发表时间：
2018-01-01
影响因子：
4
通讯作者：
Xu, Shuyun
中科院分区：
文献类型：
--
作者：
Li, Jingfeng;Yang, Liang;Xu, Shuyun
为了开发能够诱导快速骨再生的生物活性骨移植材料，采用溶胶-凝胶浸渍法将锶取代纳米羟基磷灰石（SrHA） （Sr浓度分别为0%、10%、40%和100%）涂覆在真骨陶瓷（TBC）基质上，合成了一种新型生物材料。所有涂层TBC支架都保留了固有的天然小梁结构、孔隙率和抗压强度，同时在基底表面具有微/纳米形貌的SrHA层。随着锶含量的增加，沉积的磷灰石晶体尺寸增大，颗粒密度稀疏，但在所有条件下均观察到独特的HA晶相。经修饰的TBC支架可显著增强MC3T3-E1成骨细胞的体外粘附、增殖和成骨分化。特别是，Sr10-TBC组（磷灰石涂层中含有10 mol% Sr2+）显示出最高的成骨效果。对双侧临界尺寸兔桡骨缺损模型进行12周的三维CT成像和组织学评价显示Sr10-TBC种植体有明显的骨形成。Sr10-TBC组新生骨面积比显著高于TBC组。此外，在植入12周期间，Sr10-TBC植入物比原始TBC植入物表现出更快的降解性。结果表明，10% SrHA涂层改性TBC可促进骨生成，是一种很有前途的骨缺损再生生物材料。
To develop bioactive bone graft materials that can induce rapid bone regeneration, a novel biomaterial was synthesized by coating true bone ceramic (TBC) substrates with strontium-substituted nano-hydroxyapatites (SrHA) (Sr concentrations of 0%, 10%, 40%, 100%) through a sol–gel dip-coating approach. All coated TBC scaffolds retained the inherent natural trabecular structure, porosity, compressive strength and simultaneously possessed a micro/nanotopography SrHA layer on the substrate surface. The dimension of the deposited crystal increased and the density of the deposited apatite particles became sparse with increasing Sr content, but a unique HA crystalline phase was observed under all conditions. The modified TBC scaffolds significantly enhanced the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 osteoblasts in vitro. Particularly, the Sr10-TBC group (10 mol% Sr2+ in apatite coating) revealed the highest osteogenic efficacy over the other groups. Three-dimensional CT imaging and histological evaluations on a bilateral critical-sized rabbit radial defect model for 12 weeks showed significant bone formation in the Sr10-TBC implants. The new bone area ratios of the Sr10-TBC group were significantly higher than that of the TBC group. Additionally, Sr10-TBC implants showed faster degradability compared with raw TBC implants during the 12 weeks of implantation. The results indicate that TBC modification with 10% SrHA coating stimulated osteogenesis and could be a promising biomaterial for future bone defect regeneration.