Adipogenic effects of piperlonguminine in 3T3-L1 cells and plasma concentrations of several amide constituents from Piper chaba extracts after treatment of mice
Adipogenic effects of piperlonguminine in 3T3-L1 cells and plasma concentrations of several amide constituents from Piper chaba extracts after treatment of mice
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DOI：
10.1007/s11418-013-0770-3
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发表时间：
2013-04
影响因子：
3.3
通讯作者：
Itadaki Yamaguchi;H. Matsuda;Hailong Zhang;M. Hamao;Chihiro Yamashita;Yuichiro Kogami;Haruka Kon'i;Megumi Murata;Seikou Nakamura;M. Yoshikawa
中科院分区：
文献类型：
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作者：
Itadaki Yamaguchi;H. Matsuda;Hailong Zhang;M. Hamao;Chihiro Yamashita;Yuichiro Kogami;Haruka Kon'i;Megumi Murata;Seikou Nakamura;M. Yoshikawa
在我们以前的研究中，据报道，来自胡椒果实的piperlonguminine可以促进3 T3-L1细胞中的脂肪生成，类似于过氧化物酶体增殖物激活受体-γ（PPARγ）激动剂曲格列酮。在本研究中，piperlonguminine在细胞中的作用模式进行了检查。阿洛格米宁增加脂联素，葡萄糖转运蛋白4和脂肪酸结合蛋白（aP 2）的mRNA水平。它还增加了PPARγ2的mRNA水平，但与曲格列酮不同，piperlonguminine在核受体辅因子测定中不直接激活PPARγ。对用piperlonguminine、胡椒碱和retrofractionalA以及果实提取物处理的小鼠的血浆进行分析，结果表明piperlonguminine的浓度高于胡椒碱和retrofractionalA的浓度，并且在体内施用提取物后胡椒碱的“曲线下面积”增加。
In our previous study, piperlonguminine from the fruit ofPiper chabawas reported to promote adipogenesis in 3T3-L1 cells like the peroxisome proliferator-activated receptor-γ (PPARγ) agonist, troglitazone. In the present study, the mode of action of piperlonguminine in cells was examined. Piperlonguminine increased mRNA levels of adiponectin, glucose transporter 4, and fatty acid-binding protein (aP2). It also increased mRNA levels of PPARγ2 but, unlike troglitazone, piperlonguminine did not activate PPARγ directly in a nuclear receptor cofactor assay. Analyses of plasma from mice treated with piperlonguminine, piperine, and retrofractamide A, and an extract of the fruit, showed that concentrations of piperlonguminine were higher than those of piperine and retrofractamide A, and that the “area-under-the-curve” of piperine increased following in vivo administration of the extract.