DNA methylation changes in cell line from β-lactoglobulin gene targeted fetus
DNA methylation changes in cell line from β-lactoglobulin gene targeted fetus
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DOI：
10.1016/j.anireprosci.2008.05.072
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Li, Ning
中科院分区：
文献类型：
--
作者：
Lin, Li;Xu, Weizhuo;Li, Ning
基因打靶联合体细胞核移植在农业和医学上具有重要的应用价值。DNA甲基化的表观遗传修饰在调节哺乳动物发育过程中的基因表达方面具有重要意义。在基因打靶过程中，供体细胞核的表观遗传状态可能会发生一系列的改变，包括同源重组、细胞选择和克隆。我们检测了6个基因(β-肌动蛋白、血管内皮生长因子、Oct4、TERT H19和Igf2)在BLG(+/-)细胞系中的DNA甲基化和重复序列art2。结果表明，在BLG(+/-)细胞系中存在广泛的DNA甲基化改变。但变化的程度不同。BLG(+/-)中血管内皮生长因子的DNA甲基化水平明显降低。这些观察表明，DNA甲基化变异可能会影响基因的表达，最终导致发育后期的异常和致死。(C)2008爱思唯尔B.V.保留所有权利。
The gene targeting combined somatic cell nuclear transfer is very useful in agriculture and medicine. Epigenetic modification of DNA by methylation is significant in regulating gene expression during mammalian development. During gene targeting, epigenetic status of donor cell nuclei may be changed in a series of processes, including homologous recombination, cell selection and cloning. We examined DNA methylation of six genes (beta-actin, VEGF, oct4, TERT H19 and Igf2) and a repetitive sequence art2 in blg(+/-) cell line from beta-lactoglobulin (BLG) gene targeted fetus and the cells used for BLG gene targeting serve as control. The results demonstrated that the widespread changes of DNA methylation were found in blg(+/-) cell line. But the degree of variation was different. DNA methylation of VEGF in blg(+/-) was noticeably decreased. These observations suggest that DNA methylation variations may impact gene expression and finally induce abnormalities and lethality in later developmental stages. (C) 2008 Elsevier B.V. All rights reserved.