Phylogenomic evidence supports past endosymbiosis, intracellular and horizontal gene transfer in Cryptosporidium parvum.
Phylogenomic evidence supports past endosymbiosis, intracellular and horizontal gene transfer in Cryptosporidium parvum.
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DOI：
10.1186/gb-2004-5-11-r88
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Kissinger JC
中科院分区：
文献类型：
--
作者：
Huang J;Mullapudi N;Lancto CA;Scott M;Abrahamsen MS;Kissinger JC
一个可能的内共生体或原核生物起源的隐孢子虫基因的分析支持这一假设，C。禾本科植物从含质体的谱系进化而来。顶复体寄生虫微小隐孢子虫是一种新兴病原体，能够引起人类和其他动物疾病以及免疫功能低下个体死亡。没有有效的治疗方法，基因组序列最近已经完成。这种寄生虫不同于其他apicomplexans在其缺乏质体细胞器，顶质体。基因转移，无论是从内共生体/供体细胞器的细胞内或从另一种生物体的水平，可以提供以前的内共生关系的证据和/或改变宿主生物体的遗传库。鉴于基因转移在真核生物进化中的重要性以及对化疗的潜在影响，鉴定隐孢子虫中转移基因的互补性是很重要的。我们已经确定了31个基因的可能质体/内共生体（n = 7）或原核生物（n = 24）的起源，使用一个基因组学的方法。这一发现支持了隐孢子虫从含质体的谱系进化而来，随后在进化过程中失去了顶质体的假设。对藻类和真细菌来源的候选基因的表达分析表明，这些基因在C.小的隐孢子虫是大量转移基因的接受者，其中许多基因是其他顶复门寄生虫所不共有的。从远源系统发育来源（如真细菌）转移的基因可能是治疗药物的潜在靶点，因为它们的系统发育距离或在宿主中缺乏同源物。转移基因在该基因组中的成功整合和表达改变了寄生虫的遗传和代谢谱。
An analysis of Cryptosporidium parvum genes of likely endosymbiont or prokaryotic origin supports the hypothesis that C. arvum evolved from a plastid-containing lineage. The apicomplexan parasite Cryptosporidium parvum is an emerging pathogen capable of causing illness in humans and other animals and death in immunocompromised individuals. No effective treatment is available and the genome sequence has recently been completed. This parasite differs from other apicomplexans in its lack of a plastid organelle, the apicoplast. Gene transfer, either intracellular from an endosymbiont/donor organelle or horizontal from another organism, can provide evidence of a previous endosymbiotic relationship and/or alter the genetic repertoire of the host organism. Given the importance of gene transfers in eukaryotic evolution and the potential implications for chemotherapy, it is important to identify the complement of transferred genes in Cryptosporidium. We have identified 31 genes of likely plastid/endosymbiont (n = 7) or prokaryotic (n = 24) origin using a phylogenomic approach. The findings support the hypothesis that Cryptosporidium evolved from a plastid-containing lineage and subsequently lost its apicoplast during evolution. Expression analyses of candidate genes of algal and eubacterial origin show that these genes are expressed and developmentally regulated during the life cycle of C. parvum. Cryptosporidium is the recipient of a large number of transferred genes, many of which are not shared by other apicomplexan parasites. Genes transferred from distant phylogenetic sources, such as eubacteria, may be potential targets for therapeutic drugs owing to their phylogenetic distance or the lack of homologs in the host. The successful integration and expression of the transferred genes in this genome has changed the genetic and metabolic repertoire of the parasite.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
7
作者：
Bankier, AT;Spriggs, HF;Dear, PH
通讯作者：
Dear, PH
影响因子：
13.8
作者：
Jeanmougin, F;Thompson, JD;Gibson, TJ
通讯作者：
Gibson, TJ
影响因子：
2
作者：
Carreno, RA;Martin, DS;Barta, JR
通讯作者：
Barta, JR
影响因子：
3.5
作者：
Henze, K;Horner, DS;Embley, TM
通讯作者：
Embley, TM