Preliminary Study on Dental Pulp Stem Cell-mediated Pulp Regeneration in Canine Immature Permanent Teeth
Preliminary Study on Dental Pulp Stem Cell-mediated Pulp Regeneration in Canine Immature Permanent Teeth
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DOI：
10.1016/j.joen.2012.10.002
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发表时间：
2013-02-01
影响因子：
4.2
通讯作者：
Ge, Lihong
中科院分区：
文献类型：
--
作者：
Wang, Yuanyuan;Zhao, Yuming;Ge, Lihong
前言：人类牙齿的健康取决于硬组织的完整性以及牙髓和牙周组织的活性，而牙髓和牙周组织负责营养供应。如果没有牙髓组织的滋养，牙齿破裂的可能性就会增加。在未成熟的恒牙中，牙根的发育也可能受到影响。本研究探讨了利用自体牙髓干细胞(DPSCS)在犬无髓模型中实现牙髓再生的可能性。方法：建立Beagle年轻恒切牙牙髓摘除根管预备无髓动物模型。从拔除的第一磨牙中获取自体DPSCs，并进行体外扩增以获得更多的细胞。采用与人DPSCs相同的方法，对犬DPSCs(CDPSCs)进行体内外生物学特性分析。以明胶海绵为支架，将cDPSCs移植到无髓根管内，通过放射学和组织学分析评价牙根发育情况。结果：体外和体内均成功分离鉴定出具有快速增殖、多向分化能力和发展潜力的cDPSCs。DPSCs以明胶海绵为支架植入无髓根管内后，可形成含有血管和牙本质样组织的牙髓样组织。同时观察到根管壁增厚。结论：利用干细胞介导的组织工程技术实现未成熟牙牙髓再生是可行的。(J Endod 2013；39：195-201)
Introduction: The health of human teeth depends on the integrity of the hard tissue and the activity of the pulp and periodontal tissues, which are responsible for nutritional supply. Without the nourishing of the pulp tissue, the possibility of tooth fracture can increase. In immature permanent teeth, root development may be influenced as well. This study explored the potential of using autologous dental pulp stem cells (DPSCs) to achieve pulp regeneration in a canine pulpless model. Methods: The establishment of the pulpless animal model involved pulp extirpation and root canal preparation of young permanent incisor teeth in beagles. Autologous DPSCs were obtained from extracted first molars and expanded ex vivo to obtain a larger number of cells. The biological characteristics of canine DPSCs (cDPSCs) were analyzed both in vitro and in vivo by using the same method as used in human DPSCs. cDPSCs were transplanted into the pulpless root canal with Gelfoam as the scaffold, and root development was evaluated by radiographic and, histologic analyses. Results: cDPSCs with rapid proliferation, multiple differentiation capacity, and development potential were successfully isolated and identified both in vitro and in vivo. After they were transplanted into the pulpless root canal with Gelfoam as the scaffold, DPSCs were capable of generating pulp-like tissues containing blood vessels and dentin-like tissue. Thickening of the root canal wall was also observed. Conclusions: This study demonstrates the feasibility of using stem cell mediated tissue engineering to realize pulp regeneration in immature teeth. (J Endod 2013;39:195-201)