De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response
De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response
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DOI：
10.1016/j.ygeno.2012.02.002
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发表时间：
2012-04-01
期刊：
影响因子：
4.4
通讯作者：
Zhang, Chuan-Xi
中科院分区：
文献类型：
--
作者：
Bao, Yan-Yuan;Wang, Ying;Zhang, Chuan-Xi
褐飞虱（Nilaparvata lugens，BPH）是亚洲最严重的水稻害虫。在这项研究中，我们对BPH肠道进行了全转录组分析。我们获得了超过2600万个测序读段，然后将其组装成53，553个平均大小为388 bp的单基因。基于与NCBI中可用的核苷酸序列的相似性搜索，BPH丝氨酸特异性转录组分析鉴定了21，405个序列。组装的序列用基因描述、基因本体和直链组术语簇进行注释。消化、防御和异源物质代谢相关基因在褐飞虱肠道中大量表达。其中包括33个消化相关基因、25个免疫应答基因和27个解毒相关基因。我们通过实时荧光定量PCR分析研究了不同组织中转录水平的基因表达模式，结果显示，一些基因具有前列腺素特异性表达，这暗示了它们在BPH管理中的潜在意义。(C)2012 Elsevier Inc. All rights reserved.
The brown planthopper (Nilaparvata lugens, BPH) is the most serious rice plant pests in Asia. In this study, we performed transcriptome-wide analysis on BPH intestine. We obtained more than 26 million sequencing reads that were then assembled into 53,553 unigenes with a mean size of 388 bp. Based on similarity search with the nucleotide sequences available at NCBI, BPH intestine-specific transcriptome analysis identified 21,405 sequences. Assembled sequences were annotated with gene description, gene ontology and clusters of orthologous group terms. The digestion-, defense- and xenobiotic metabolism-related genes were abundantly detected in the transcripts from BPH intestine. Many novel genes including 33 digestion-related genes, 25 immune responsive genes and 27 detoxification-related genes are first reported here. We investigated the gene expression patterns at the transcript levels in different tissues by quantitative real-time PCR analysis, which revealed that some genes had intestine-specific expression, implicating their potential significance for BPH management. (C) 2012 Elsevier Inc. All rights reserved.