Translation initiation factor (iso) 4E interacts with BTF3, the subunit of the nascent polypeptide-associated complex
Translation initiation factor (iso) 4E interacts with BTF3, the subunit of the nascent polypeptide-associated complex
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DOI：
10.1016/j.gene.2004.11.030
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发表时间：
2005-01-31
期刊：
影响因子：
3.5
通讯作者：
Freire, MA
中科院分区：
文献类型：
--
作者：
Freire, MA
用来自拟南芥的翻译起始因子eIF（iso）4 E进行的双杂交筛选鉴定了编码2型脂氧合酶的克隆[Freire，M.A.，例如，2000.植物脂氧合酶2是一种翻译起始因子4 E结合蛋白。Plant Molecular Biology 44，129-140]，和编码哺乳动物BTF 3因子同源物的三个cDNA克隆，所述同源物是新生多肽相关复合物（NAC）的β亚基。在这里，我们报告翻译起始因子eIF（iso）4 E和AtBTF 3之间的相互作用。AtBTF 3蛋白能够与小麦起始因子eIF 4 E和eIF（iso）4 E相互作用。AtBTF 3含有与大多数4 E结合蛋白上发现的原型基序相关的序列，并且在双杂交干扰测定中与翻译起始因子eIF（iso）4G竞争eIF 4（iso）4 E结合。这些发现提供了翻译起始机制和新生多肽链出现之间的分子联系。(C)2004 Elsevier B. V.保留所有权利。
A two-hybrid screen with the translation initiation factor, eIF(iso)4E from Arabidopsis, identified a clone encoding a lipoxygenase type 2 [Freire, M.A., et al., 2000. Plant lipoxygenase 2 is a translation initiation factor-4E-binding protein. Plant Molecular Biology 44, 129-140], and three cDNA clones encoding the homologue of the mammalian BTF3 factor, the beta subunit of the nascent polypeptide-associated complex (NAC). Here we report on the interaction between the translation initiation factor eIF(iso)4E and AtBTF3. AtBTF3 protein is able to interact with the wheat initiation factors eIF4E and eIF(iso)4E. AtBTF3 contains a sequence related to the prototypic motif found on most of the 4E-binding proteins, and competes with the translation initiation factor eIF(iso)4G for eIF4(iso)4E binding, in a two hybrid interference assay. These findings provide a molecular link between the translation initiation mechanism and the emergence of the nascent polypeptide chains. (C) 2004 Elsevier B.V. All rights reserved.