Cryptococcus neoformans mitochondrial genomes from serotype A and D strains do not influence virulence
Cryptococcus neoformans mitochondrial genomes from serotype A and D strains do not influence virulence
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DOI：
10.1007/s00294-004-0521-9
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发表时间：
2004-10-01
期刊：
影响因子：
2.5
通讯作者：
Perfect, JR
中科院分区：
文献类型：
--
作者：
Toffaletti, DL;Nielsen, K;Perfect, JR
新型隐球菌是一种包囊致病酵母菌，可引起脑膜脑炎。遗传学研究中的两种主要菌株，血清型A H99和血清型D JEC21，在毒力方面具有显著差异。由于它已被证明，线粒体基因的表达是突出的感染部位和血清型A和D菌株之间的线粒体基因结构和调节有显着差异，本研究使用AD杂交移动血清型A和D线粒体不同的基因组影响。当血清型D MATa菌株参与交配杂交时，存在线粒体DNA的单亲传递，但是对于血清型A MATa菌株，线粒体DNA可以从任何亲本遗传，并且也可能发生线粒体基因组中的重组。在血清型A和D菌株之间的毒力研究中，发现在中枢神经系统中生长的毒力复合物的主要遗传控制在核DNA中编码，而不是通过线粒体DNA。
Cryptococcus neoformans is an encapsulated pathogenic yeast producing meningoencephalitis. Two primary strains in genetic studies, serotype A H99 and serotype D JEC21, possess dramatic differences in virulence. Since it has been shown that mitochondrial gene expression is prominent at the site of the infection and there are significant differences between mitochondrial gene structure and regulation between the serotype A and D strains, this study used AD hybrids to move serotype A and D mitochondria under different genomic influences. When the serotype D MATa strain is involved in the mating crosses, there is uniparental transmission of mitochondrial DNA, but with the serotype A MATa strain, mitochondrial DNA can be inherited from either parent and recombination in the mitochondrial genome may also occur. In virulence studies between serotype A and D strains, it was found that the primary genetic control of the virulence composite for growth in the central nervous system is encoded in the nuclear DNA and not through mitochondrial DNA.