Cholesterol-lowering effect of rice bran protein containing bile acid-binding proteins
Cholesterol-lowering effect of rice bran protein containing bile acid-binding proteins
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DOI：
10.1080/09168451.2014.978260
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
Jilite Wang;M. Shimada;Y. Kato;Mio Kusada;S. Nagaoka
中科院分区：
文献类型：
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作者：
Jilite Wang;M. Shimada;Y. Kato;Mio Kusada;S. Nagaoka
众所周知，膳食植物蛋白可以降低血清胆固醇水平。米糠是碾米的副产品，是蛋白质的良好来源。本研究探讨了是否喂养大鼠高胆固醇饮食含有10%的米糠蛋白（RBP）的10天影响胆固醇代谢。与那些喂食酪蛋白的大鼠相比，喂食RBP的大鼠血清总胆固醇水平较低，粪便类固醇（如胆固醇和胆汁酸）的排泄量增加。体外实验表明，与酪蛋白相比，RBP与牛磺胆酸盐结合强烈，并抑制胆固醇的胶束溶解性。此外，通过与胆酸缀合的层析柱洗脱RBP的胆汁酸结合蛋白，并且使用MALDI-TOF质谱分析鉴定其中一个为假设蛋白OsJ_13801（NCBI登录号EAZ 29742）。这些结果表明，RBP的降胆固醇作用可能是由胆汁酸结合蛋白引起的。图示摘要米糠蛋白的胆汁酸结合蛋白质（例如假设蛋白质OsJ_13801）通过抑制胆固醇在肠道中的胶束溶解度而表现出降低胆固醇的作用。
Dietary plant protein is well known to reduce serum cholesterol levels. Rice bran is a by-product of rice milling and is a good source of protein. The present study examined whether feeding rats a high-cholesterol diet containing 10% rice bran protein (RBP) for 10 d affected cholesterol metabolism. Rats fed dietary RBP had lower serum total cholesterol levels and increased excretion of fecal steroids, such as cholesterol and bile acids, than those fed dietary casein. In vitro assays showed that RBP strongly bound to taurocholate, and inhibited the micellar solubility of cholesterol, compared with casein. Moreover, the bile acid-binding proteins of the RBP were eluted by a chromatographic column conjugated with cholic acid, and one of them was identified as hypothetical protein OsJ_13801 (NCBI accession No. EAZ29742) using MALDI-TOF mass spectrometry analysis. These results suggest that the hypocholesterolemic action of the RBP may be caused by the bile acid-binding proteins. Graphical Abstract The bile acid-binding proteins (e.g. hypothetical protein OsJ_13801) of rice bran protein express the hypocholesterolemic action via inhibiting the micellar solubility of cholesterol in the intestine.