A Nucleic Acid Sequence That is Catalytically Active in Both RNA and TNA Backbones.
A Nucleic Acid Sequence That is Catalytically Active in Both RNA and TNA Backbones.
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DOI：
10.1021/acssynbio.2c00479
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发表时间：
2022-10
影响因子：
4.7
通讯作者：
D. Wei;Yueyao Wang;Dongfan Song;Zehui Zhang;Juan Wang;Jia-Yu Chen;Zhe Li;Hanyang Yu
中科院分区：
文献类型：
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作者：
D. Wei;Yueyao Wang;Dongfan Song;Zehui Zhang;Juan Wang;Jia-Yu Chen;Zhe Li;Hanyang Yu
Threose nucleic acid (TNA) is considered a potential RNA progenitor due to its chemical simplicity, base pairing property, and capability of folding into a functional tertiary structure. However, it is unknown whether the functional property can be maintained during transition from TNA to RNA. Here, we use a toggle in vitro selection to identify nucleic acid catalyst sequences that are active in both TNA and RNA backbones. One such nucleic acid enzyme with exchangeable backbone (CAMELEON) catalyzes an RNA cleavage reaction when prepared as TNA (T) and RNA (R). Further biochemical characterization reveals that CAMELEON R and T exhibit different catalytic behaviors such as rate enhancement and magnesium dependence. Structural probing and mutagenesis experiments suggest that they likely fold into distinct tertiary structures. This work demonstrates that the catalytic activity can be preserved during backbone transition from TNA to RNA and provides further experimental support for TNA as an RNA precursor in evolution.
