Efficiency of MS2 phage and Qβ phage removal by membrane filtration in water treatment: Applicability of real-time RT-PCR method
Efficiency of MS2 phage and Qβ phage removal by membrane filtration in water treatment: Applicability of real-time RT-PCR method
复制标题
水处理中膜过滤去除 MS2 噬菌体和 Qβ 噬菌体的效率：实时 RT-PCR 方法的适用性
DOI：
10.1016/j.memsci.2008.09.044
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发表时间：
2009
影响因子：
9.5
通讯作者：
C. Gantzer
中科院分区：
文献类型：
--
作者：
J. Langlet;L. Ogorzaly;J. Schrotter;C. Machinal;F. Gaboriaud;J. Duval;C. Gantzer
目前，MS2噬菌体被用作评价水处理过程中过滤膜对致病性病毒去除效果的替代物。噬菌体去除通常是通过比较PFU法在渗透液和饲料溶液中的感染单位数量来定义的。由于可能发生病毒聚集，这种方法可能导致对病毒去除的估计过高。在这项研究中，我们报告了水处理技术中使用的各种常规膜对MS2和Qβ噬菌体悬浮液的去除效率。这些噬菌体——已知在静电电荷和疏水性方面表现出明显的界面特征——被用于病毒颗粒聚集可以忽略不计的条件下。在这种条件下，快速、易于操作的分子生物学方法，即实时RT-PCR，用于测量病毒去除率的可行性得到了明确的解决。并将该方法与传统方法的计算结果进行了比较。根据过滤结果，Qβ噬菌体被清除的程度低于MS2噬菌体，这里考虑的所有膜都是如此。因此，分析质疑选择MS2噬菌体作为最坏条件下表征膜病毒去除倾向的最佳替代品。
MS2 phage is currently used as a surrogate to evaluate pathogenic virus removal efficiency by filtration membrane during water treatment. Phage removal is commonly defined upon comparing number of infectious units in permeate with that in feed solution by PFU method. This method may lead to overestimation of the virus removal because of possible occurrence of viruses aggregation. In this study, we report the removal efficiencies of various conventional membranes used in water treatment technology, with respect to suspensions of MS2 and Qβ bacteriophages. These phages – known to exhibit distinct interfacial characteristics in terms of electrostatic charge and degree of hydrophobicity – were used in conditions where viral particle aggregation is negligible. Under such conditions, the feasibility of fast, easy to handle, molecular biology method, i.e. real-time RT-PCR, for measuring virus removal rates is clearly addressed. Comparison of the results provided by this method with those obtained via more traditional procedure is given. On the basis of the filtration results, it is shown that Qβ phages are eliminated to a lower extent than MS2 phages, this being the case for all membranes considered here. Accordingly, the analysis questions the choice of MS2 phage as the best surrogate classically used for characterizing membrane virus removal propensity in worst possible conditions.