EXPRESSION AND PURIFICATION OF CLOSTRIDIUM-PERFRINGENS BETA-TOXIN GLUTATHIONE-S-TRANSFERASE FUSION PROTEIN
EXPRESSION AND PURIFICATION OF CLOSTRIDIUM-PERFRINGENS BETA-TOXIN GLUTATHIONE-S-TRANSFERASE FUSION PROTEIN
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DOI：
10.1111/j.1574-6968.1995.tb07731.x
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发表时间：
1995-08-01
影响因子：
2.1
通讯作者：
ANDRESSON, OS
中科院分区：
文献类型：
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作者：
STEINPORSDOTTIR, V;FRIDRIKSDOTTIR, V;ANDRESSON, OS
从C型产气荚膜梭菌中克隆β-毒素基因，并在大肠杆菌中表达为谷胱甘肽S-转移酶融合蛋白。测定DNA序列并与B型序列进行比较。在蛋白质编码序列中发现两个核苷酸差异，导致两种蛋白质之间存在一个氨基酸差异。纯化的β-毒素融合蛋白对小鼠无毒性，但针对其产生的兔抗血清中和了C.小鼠中的C型产气荚膜杆菌培养滤液。
The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid difference between the two proteins. The purified beta-toxin fusion protein is not toxic in mice, but rabbit antiserum raised against it neutralises the toxic effect of C. perfringens type C culture filtrate in mice.