Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device
Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device
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在微流体装置中利用间质流重建肝组织结构
DOI：
10.1007/978-1-4939-8961-4_15
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发表时间：
2018
影响因子：
--
通讯作者：
Ryo Sudo
中科院分区：
文献类型：
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作者：
Tokuno Junko;Chen-Yoshikawa Toyofumi F.;Nakao Megumi;Matsuda Tetsuya;Date Hiroshi;Ryo Sudo
三维肝组织结构的构建对于体外肝脏组织工程非常重要，因为肝细胞的三维培养对于维持肝脏特异性功能至关重要。虽然传统的3D培养方法可用于构建3D肝组织结构，但需要精确控制培养微环境以在体外构建更多的生理组织。微流体技术的最新进展使我们能够利用微流体装置进行肝细胞培养，这为创建更多的肝脏生理3D培养模型打开了大门。在这里，我们描述了使用微流体装置构建肝脏组织结构的方法，该装置具有带有相邻微通道的3D凝胶区域。将原代大鼠肝细胞接种到微流体装置中的微通道中。然后在间质流条件下培养细胞，从而构建3D组织结构。
Construction of three-dimensional (3D) hepatic tissue structures is important for in vitro tissue engineering of the liver, because 3D culture of hepatocytes is critical for the maintenance of liver-specific functions. Although conventional 3D culture methods are useful for constructing 3D hepatic tissue structures, the precise control of culture microenvironments is required to construct more physiological tissues in vitro. Recent advances in microfluidics technologies have allowed us to utilize microfluidic devices for hepatic cell culture, which opened the door for creating more physiological 3D culture models of the liver. Here, we describe the method for the construction of hepatic tissue structures using a microfluidic device which has a 3D gel region with adjacent microchannels. Primary rat hepatocytes are seeded into a microchannel in a microfluidic device. The cells are then cultured in interstitial flow conditions, which leads to the construction of 3D tissue structures.