INTRACELLULAR-RECORDING FROM PUTATIVE DOPAMINE-CONTAINING NEURONS IN THE VENTRAL TEGMENTAL AREA OF TSAI IN A BRAIN SLICE PREPARATION
INTRACELLULAR-RECORDING FROM PUTATIVE DOPAMINE-CONTAINING NEURONS IN THE VENTRAL TEGMENTAL AREA OF TSAI IN A BRAIN SLICE PREPARATION
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DOI：
10.1016/0165-0270(89)90005-8
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发表时间：
1989-05-01
影响因子：
3
通讯作者：
BRODIE, MS
中科院分区：
文献类型：
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作者：
MUELLER, AL;BRODIE, MS
制备了大鼠中脑含有腹侧被盖区（VTA）和黑质的冠状面切片。从假定的VTA中含有多巴胺（DA）的神经元获得了稳定的细胞内记录。沉默的细胞和自发活跃的细胞都遇到了；自发活动的神经元以极其规律的类似心脏起搏器的方式放电。这些神经元的静息膜电位范围为- 45至- 75 mV，输入电阻范围为80-400 MΩ。含da的VTA神经元在超极化电流脉冲下表现出明显的异常整流。在沐浴介质中应用DA或gababagon剂巴氯芬可抑制自发放电，有时伴有膜超极化。多巴胺对神经元输入电阻的改变不一致，巴氯芬一般会降低神经元输入电阻。
Coronal slices of rat mesencephalon containing the ventral tegmental area of Tsai (VTA) and the substantia nigra were prepared. Stable intracellular recordings were obtained from presumed dopamine (DA)-containing neurons in the VTA. Both silent and spontaneously active cells were encountered; spontaneously active neurons fired in an extremely regular pacemaker-like fashion. These neurons had resting membrane potentials ranging from −45 to −75 mV and input resistances ranging from 80–400 MΩ. DA-containing neurons in the VTA demonstrated marked anomalous rectification in response to hyperpolarizing current pulses. Application of DA or the GABABagonist, baclofen, to the bathing medium produced suppression of spontaneous firing, sometimes accompanied by membrane hyperpolarization. Neuronal input resistance was not changed consistently by DA and was generally reduced by baclofen.