The pan-genome of Splendidus clade species in the family Vibrionaceae: Insights into evolution, adaptation, and pathogenicity
The pan-genome of Splendidus clade species in the family Vibrionaceae: Insights into evolution, adaptation, and pathogenicity
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DOI：
10.1111/1462-2920.16209
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发表时间：
2022-09-25
影响因子：
5.1
通讯作者：
Sawabe, Tomoo
中科院分区：
文献类型：
--
作者：
Jiang, Chunqi;Kasai, Hisae;Sawabe, Tomoo
Splendidus分支是弧菌科中最大的分支，其成员常与海洋动物的死亡有关，造成巨大的经济损失。然而，其致病性和毒性的分子基础在很大程度上仍然未知。特别是，完整的基因组序列的Splendidus进化枝物种很少登记，这是一个障碍，预测核心和/或独特的基因负责他们的适应性和致病性，并进行细规模的元转录组在细菌感染到他们的主机。在本研究中，我们获得了Splendidus分支中所有模式菌株的完整基因组，并发现：（1）不同的基因组大小，（4.4 - 5.9Mb），其中迟缓弧菌最大，且大多数菌株都含有几个大质粒，这可能是由于游动体元件的不同特征所致;（2）Splendidus分支由19个种组成，除V.cortegadensis外，还划分了3个亚分支（SC），其中15个与之关系最密切的成员为SC 1;（3）不同的碳水化合物降解偏好可能是环境适应的结果;（4）对毒力因子（VF）的广泛预测揭示了核心和物种独特的VF基因。
The Splendidus clade is the largest clade in Vibrionaceae, and its members are often related to mortality of marine animals with huge economic losses. The molecular bases of their pathogenicity and virulence, however, remain largely unknown. In particular, the complete genome sequences of the Splendidus clade species are rarely registered, which is one of the obstacles to predict core and/or unique genes responsible for their adaptation and pathogenicity, and to perform a fine scale meta-transcriptome during bacterial infection to their hosts. In this study, we obtained the complete genomes of all type strains in the Splendidus clade and revealed that (1) different genome sizes (4.4-5.9 Mb) with V. lentus the biggest and most of them had several big plasmids, likely because of the different features on mobilome elements; (2) the Splendidus clade consists of 19 species except V. cortegadensis, and 3 sub-clades (SC) were defined with the 15 most closely related members as SC1; (3) different carbohydrate degradation preferences may be the result of environmental adaptation; and (4) a broad prediction of virulence factors (VFs) revealed core and species unique VF genes.