IMMUNOCYTOCHEMISTRY OF CELL-SURFACE HEPARAN-SULFATE PROTEOGLYCAN IN MOUSE-TISSUES - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
IMMUNOCYTOCHEMISTRY OF CELL-SURFACE HEPARAN-SULFATE PROTEOGLYCAN IN MOUSE-TISSUES - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1177/35.10.2957423
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发表时间：
1987-10-01
影响因子：
3.2
通讯作者：
BERNFIELD, M
中科院分区：
文献类型：
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作者：
HAYASHI, K;HAYASHI, M;BERNFIELD, M
NMuMG小鼠乳腺上皮细胞细胞表面的蛋白聚糖的核心蛋白具有乙酰肝素和硫酸软骨素链，并被单克隆抗体281-2识别。使用这种抗体和过氧化物酶-抗过氧化物酶染色技术在成年小鼠组织中，我们发现，该抗体识别的抗原在一个高度限制的分布，染色的各种上皮细胞，但没有来自胚胎中胚层或神经嵴的细胞。抗体不能染色任何基质（间充质）或神经元细胞，浆细胞和Leydig细胞除外。鳞状上皮和移行上皮在其整个表面上染色强烈，而立方上皮和柱状上皮仅在基底细胞的侧面中度染色。在经历生理再生的鳞状和移行上皮组织内（例如，表皮），最表面和分化的细胞类型不能染色。在腺体和分支上皮内（例如，胰腺），分泌性肺泡细胞不能染色。当通过电子显微镜进行评价时，在质膜上，特别是在侧表面上观察到染色的颗粒状沉积物，但在细胞或基底膜内未观察到。这些结果表明，在成人组织中，这种富含硫酸乙酰肝素的蛋白聚糖的核心蛋白几乎只在上皮细胞表面表达。随着细胞变得成熟或高度分化，表达似乎丧失。
The core protein of the proteoglycan at the cell surface of NMuMG mouse mammary epithelial cells bears both heparan and chondroitin sulfate chains and is recognized by the monoclonal antibody 281-2. Using this antibody and the peroxidase-antiperoxidase staining technique in adult mouse tissues, we found that the antibody recognizes the antigen in a highly restricted distribution, staining a variety of epithelial cells but no cells derived from embryonic mesoderm or neural crest. The antibody fails to stain any stromal (mesenchymal) or neuronal cells, with the exception of plasma cells and Leydig cells. Squamous and transitional epithelia stain intensely over their entire surfaces, whereas cuboidal and columnar epithelia stain moderately and only at the lateral surface of the basal cells. Within squamous and transitional epithelial tissues that undergo physiological regeneration (e.g., epidermis), the most superficial and differentiated cell types fail to stain. Within glandular and branched epithelia (e.g., pancreas), the secretory alveolar cells fail to stain. When evaluated by electron microscopy, granular deposits of stain are seen on the plasma membrane, especially on lateral surfaces, but none are noted within the cells or the basement membrane. These results indicate that in adult tissues the core protein of this heparan sulfate-rich proteoglycan is expressed almost exclusively at epithelial cell surfaces. Expression appears to be lost as the cells become either mature or highly differentiated.