Decreased stress-induced depression-like behavior in lactating rats is associated with changes in the hypothalamic-pituitary-adrenal axis, brain monoamines, and brain amino acid metabolism
Decreased stress-induced depression-like behavior in lactating rats is associated with changes in the hypothalamic-pituitary-adrenal axis, brain monoamines, and brain amino acid metabolism
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DOI：
10.1080/10253890.2019.1584179
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发表时间：
2019-07-04
影响因子：
2.3
通讯作者：
Furuse, Mitsuhiro
中科院分区：
文献类型：
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作者：
Hamada, Mizuki;Nishigawa, Takuma;Furuse, Mitsuhiro
哺乳期抑郁样行为可能与下丘脑-垂体-肾上腺（HPA）轴、脑单胺和脑氨基酸代谢的变化有关。本研究探讨了应激和哺乳期对大鼠行为、HPA轴活性、脑内单胺类物质和脑内游离氨基酸代谢的影响。将大鼠分为四组：（1）对照泌乳（n=6），（2）应激泌乳（n=6），（3）对照处女（n=7），和（4）应激处女（n=7），并从出生后第1天（PND）开始限制30分钟，共10次（隔日一次）。在PND 10测定强迫游泳试验（FST）中的抑郁样行为，在PND 19测定血浆皮质酮以及前额叶皮层和下丘脑中单胺和L-氨基酸（包括β-丙氨酸、γ-氨基丁酸、胱硫醚、3-甲基组氨酸和牛磺酸）的浓度。血浆皮质酮浓度，测量约束应力后，显着较高的压力组，与对照组相比，但有没有显着差异之间的控制和应力泌乳组。抑郁样行为（不动）在FST是显着较低的哺乳组，与处女组。压力增加多巴胺和谷氨酸，并减少苏氨酸和甘氨酸的浓度在下丘脑。此外，3-甲氧基-4-羟基苯乙二醇（MHPG），苏氨酸和鸟氨酸的浓度在前额叶皮层显着高于泌乳组相比，处女组。血浆皮质酮浓度，单胺和氨基酸代谢的变化可能与应激诱导的抑郁样行为在哺乳rates.Lay summaryThis研究表明，减少抑郁样行为在哺乳期，相对于处女大鼠，与单胺和氨基酸代谢的变化在大脑的前额皮质。此外，应激对下丘脑单胺和氨基酸代谢的影响显著，可能与神经内分泌应激轴活动和皮质酮分泌有关。这项研究表明，应激诱导的抑郁样行为可能与应激轴，脑单胺和脑氨基酸代谢的几个变化。这些参数与哺乳期大鼠抑郁样行为减弱有关。
Depression-like behavior during lactation may relate to changes in the hypothalamic-pituitary-adrenal (HPA) axis, brain monoamines, and brain amino acid metabolism. This study investigated how the behavior, HPA axis activity, brain monoamines, and brain free amino acid metabolism of rats were changed by stress or lactation period. Rats were separated into four groups: (1) control lactating (n=6), (2) stress lactating (n=6), (3) control virgin (n=7), and (4) stress virgin (n=7) and restrained for 30min a total of ten times (once every other day) from postnatal day (PND) 1. Depression-like behavior in the forced swimming test (FST) on PND 10 and concentration of corticosterone in plasma, as well as monoamines and L-amino acids including beta-alanine, gamma-aminobutyric acid, cystathionine, 3-methyl-histidine and taurine in the prefrontal cortex and hypothalamus on PND 19 were measured. The plasma corticosterone concentration, measured just after restraint stress, was significantly higher in the stress groups, versus the control groups, but there were no significant differences between control and stress lactating groups. Depression-like behavior (immobility) in the FST was significantly lower in the lactating groups, versus the virgin groups. Stress enhanced dopamine and glutamate, and decreased threonine and glycine concentrations in the hypothalamus. In addition, 3-methoxy-4-hydroxyphenylglycol (MHPG), threonine and ornithine concentrations in the prefrontal cortex were significantly higher in the lactating groups compared with the virgin groups. Changes in plasma corticosterone concentration, monoamine, and amino acid metabolism may relate to stress-induced depression-like behavior in lactating rats.Lay summaryThis study revealed that reduced depression-like behavior in lactating, relative to virgin rats, was associated with changes in monoamine and amino acid metabolism in the prefrontal cortex of the brain. In addition, the effect of stress on monoamine and amino acid metabolism is prominently observed in the hypothalamus and may be related to neuroendocrine stress axis activity and secretion of corticosterone. This study suggested that stress-induced depression-like behavior may be associated with several changes in the stress axis, brain monoamines, and brain amino acid metabolism. These parameters were associated with attenuated depression-like behavior in lactating rats.