Neurotransmitter release at the thalamocortical synapse instructs barrel formation but not axon patterning in the somatosensory cortex.
Neurotransmitter release at the thalamocortical synapse instructs barrel formation but not axon patterning in the somatosensory cortex.
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DOI：
10.1523/jneurosci.0343-12.2012
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发表时间：
2012-05-02
期刊：
影响因子：
--
通讯作者：
Gaspar P
中科院分区：
文献类型：
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作者：
Narboux-Nême N;Evrard A;Ferezou I;Erzurumlu RS;Kaeser PS;Lainé J;Rossier J;Ropert N;Südhof TC;Gaspar P
为了评估突触神经递质释放对神经回路发育的影响，我们分析了丘脑或皮质消融RIM 1和RIM 2蛋白（控制突触囊泡融合）后桶状皮质的形成。丘脑特异性RIMs缺失使神经传递效率降低67%。一个barrelless表型被发现与分离的影响，突触前和突触后的细胞成分的桶。突触前，丘脑皮层轴突形成了一个正常的晶须地图，而突触后的细胞结构的第四层神经元被改变为多刺的星状神经元均匀分布，他们的树突树是对称的。引人注目的是，皮层特异性的RIM基因缺失并没有改变桶的发展。具有丘脑特异性RIM缺失的成年小鼠表现出缺乏活动触发的立即早期基因表达和改变的感觉相关行为。因此，有效的突触释放是需要在丘脑皮质，但不是在皮质突触的建设须桶地图和有效的感觉功能。
To assess the impact of synaptic neurotransmitter release on neural circuit development, we analyzed barrel cortex formation after thalamic or cortical ablation of RIM1 and RIM2 proteins, which control synaptic vesicle fusion. Thalamus-specific deletion of RIMs reduced neurotransmission efficacy by 67%. A barrelless phenotype was found with a dissociation of effects on the presynaptic and postsynaptic cellular elements of the barrel. Presynaptically, thalamocortical axons formed a normal whisker map, whereas postsynaptically the cytoarchitecture of layer IV neurons was altered as spiny stellate neurons were evenly distributed and their dendritic trees were symmetric. Strikingly, cortex-specific deletion of the RIM genes did not modify barrel development. Adult mice with thalamic-specific RIM deletion showed a lack of activity-triggered immediate early gene expression and altered sensory-related behaviors. Thus, efficient synaptic release is required at thalamocortical but not at corticocortical synapses for building the whisker to barrel map and for efficient sensory function.