A semiquantitative culture method for identifying intravenous-catheter-related infection.
A semiquantitative culture method for identifying intravenous-catheter-related infection.
复制标题
一种识别静脉导管相关感染的半定量培养方法。
DOI：
--
复制
发表时间：
1977
影响因子：
158.5
通讯作者：
H. W. Sarafin
中科院分区：
文献类型：
--
作者：
D. Maki;C. Weise;H. W. Sarafin
我们评估了一种半定量培养技术，用于识别静脉导管引起的感染：将导管段滚动通过血琼脂。将该方法与肉汤培养进行了比较。在研究的250根导管中，225根（90%）在半定量培养上有低密度定植（平板上少于15个菌落），尽管其中49根（19.6%）在肉汤或平板上生长了一些微生物。这些导管均未导致败血症。25个导管（10%）生长大于或等于15个菌落的半定量技术，大多数融合生长。败血症源自其中4根导管（P = 0.008）。在37根暴露于远处感染灶细菌的导管中，4根在肉汤中产生了匹配的生长，而在半定量培养中没有一根与血液分离株一致。局部炎症与高密度定植半定量培养相关（P <0.001）。半定量技术区分感染（大于或等于15个菌落）和污染，在诊断导管相关败血症方面比肉汤中的导管培养更具特异性。
We evaluated a semiquantitative culture technic for identifying infection due to intravenous catheters: rolling the catheter segment across blood agar. This method was compared to broth culture. Of 250 catheters studied, 225 (90%) had low-density colonization on semiquantitative culture (less than 15 colonies on the plate) although 49 (19.6%) of these grew some organisms in broth or on the plate. None of these catheters led to septicemia. Twenty-five catheters (10%) grew greater than or equal to 15 colonies by the semiquantitative technic; most gave confluent growth. Septicemia originated from four of these catheters (P = 0.008). Of 37 catheters exposed to bacteremias from distant foci of infection, four yielded matching growth in broth, whereas none were concordant with the blood isolate on semiquantitative culture. Local inflammation was associated with high-density colonization semiquantitative culture (P less than 0.001). The semiquantitative technic distinguishes infection (greater than or equal to 15 colonies) from contamination and is more specific in diagnosis of catheter-related septicemia than culture of the catheter in broth.