β-catenin controls hair follicle morphogenesis and stem cell differentiation in the skin
β-catenin controls hair follicle morphogenesis and stem cell differentiation in the skin
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DOI：
10.1016/s0092-8674(01)00336-1
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发表时间：
2001-05-18
期刊：
影响因子：
64.5
通讯作者：
Birchmeier, W
中科院分区：
文献类型：
--
作者：
Huelsken, J;Vogel, R;Birchmeier, W
β-连环蛋白是Wnt/wingless信号传导中的必需分子，其控制胚胎发生中的决定性步骤。为了研究β-连环蛋白在皮肤发育中的作用，我们使用Cre/loxP技术在表皮和毛囊中引入该基因的条件突变。当β-连环蛋白在胚胎发育过程中发生突变时，产生毛囊的基板的形成就被阻断了。我们发现β-连环蛋白在遗传上是必需的，在基板形成中，在虎斑/无绒毛的下游和BMP和SHH的上游。如果β-连环蛋白在毛囊形成后被删除，头发在第一个毛发周期后就完全脱落了。进一步的分析表明，β-连环蛋白对于皮肤干细胞的命运决定是必不可少的：在β-连环蛋白不存在的情况下，干细胞倾向于分化成滤泡角质形成细胞，而是采取表皮命运.
beta -Catenin is an essential molecule in Wnt/wingless signaling, which controls decisive steps in embryogenesis. To study the role of beta -catenin in skin development, we introduced a conditional mutation of the gene in the epidermis and hair follicles using Cre/loxP technology. When beta -catenin is mutated during embryogenesis, formation of placodes that generate hair follicles is blocked. We show that beta -catenin is required genetically downstream of tabby/downless and upstream of bmp and shh in placode formation. If beta -catenin is deleted after hair follicles have formed, hair is completely lost after the first hair cycle. Further analysis demonstrates that beta -catenin is essential for fate decisions of skin stem cells: in the absence of beta -catenin, stem cells rail to differentiate into follicular keratinocytes, but instead adopt an epidermal fate.