Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA.
Complementary DNA structure of the high molecular weight rat insulin-like growth factor binding protein (IGF-BP3) and tissue distribution of its mRNA.
复制标题
高分子量大鼠胰岛素样生长因子结合蛋白 (IGF-BP3) 的互补 DNA 结构及其 mRNA 的组织分布。
DOI：
10.1016/s0006-291x(89)80052-x
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Ling,N
中科院分区：
文献类型：
--
作者：
Shimasaki,S;Koba,A;Mercado,M;Shimonaka,M;Ling,N
在细胞外液中发现的胰岛素样生长因子(IGF)与特定的结合蛋白结合。最近从猪的卵泡液中分离到一种高分子量的胰岛素样生长因子结合蛋白(IGF-BP3)，该蛋白能抑制卵泡刺激素刺激的大鼠颗粒细胞产生雌二醇。通过分子克隆推导出猪IGF-BP3的完整一级结构。以猪的cDNA为探针，我们已经从孕马血清促性腺激素刺激的卵巢文库中分离并鉴定了大鼠IGF-BP3的编码基因。预测的氨基酸序列显示了一个由265个氨基酸组成的成熟多肽，含有18个半胱氨酸和4个潜在的天冬氨酸连接的糖基化位点。Northern分析显示，在大鼠的肝、肾、胃、心脏、肾上腺、卵巢、睾丸、脾、肺、小肠和大肠中均有一条2.6kb的单一条带，但在下丘脑和大脑皮层中该条带的含量低于检测限值。
Insulin-like growth factors (IGFs) found in extracellular fluids are bound to specific binding proteins. Recently a high molecular weight IGF-binding protein (IGF-BP3) has been isolated from porcine ovarian follicular fluid based on its inhibition of follicle stimulating hormone-stimulated estradiol production in rat granulosa cells. The complete primary structure of the porcine IGF-BP3 was deduced by molecular cloning. Using the porcine cDNA as a probe, we have now isolated and characterized cDNAs encoding rat IGF-BP3 from a pregnant mare serum gonadotropin-stimulated ovarian library. The predicted amino acid sequence revealed a mature polypeptide consisting of 265 amino acids with 18 cysteines and 4 potential Asn-linked glycosylation sites. Northern analysis of the IGF-BP3 mRNA in rat tissues showed a single 2.6 kb band in liver, kidney, stomach, heart, adrenal, ovary, testis, spleen, lung, small and large intestine in varying amounts, but the message is below the limit of detection in hypothalamus and brain cortex.