The epigenetic modifications and the anterior to posterior characterization of meiotic entry during mouse oogenesis
The epigenetic modifications and the anterior to posterior characterization of meiotic entry during mouse oogenesis
复制标题
小鼠卵子发生过程中减数分裂进入的表观遗传修饰和前后特征
DOI：
10.1007/s00418-017-1545-9
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发表时间：
2017-07-01
影响因子：
2.3
通讯作者：
Sun, Xiao-Feng
中科院分区：
文献类型：
--
作者：
Fu, Xia-Fei;Yang, Fan;Sun, Xiao-Feng
哺乳动物卵原细胞减数分裂的起始是原始生殖细胞发育为成熟卵母细胞的关键步骤。在这项研究中，表观遗传修饰和DAZL基因表达卵原细胞减数分裂进入进行了系统的调查。我们发现DAZL的表达受其启动子区域内CpG岛DNA甲基化的表观遗传调控。在减数分裂进入过程中，从11.5到16.5 dpc（交配后天数）观察到5 hmC（通常与基因表达激活相关的稳定表观遗传标记）水平持续增加。同时，组蛋白3（H3 K27 me 3）上赖氨酸27的三甲基化，通常与基因表达的抑制有关，从12.5 dpc持续增加到16.5 dpc。最后，通过将卵巢等分为代表卵巢前部、中部和后部的三个区域，并对各个区域进行免疫荧光和qRT-PCR，我们提供了进一步的证据，证明雌性生殖细胞的减数分裂进入和进展是前向后的模式。
The meiotic initiation of mammalian oogonia is a critical step during the development of primordial germ cells (PGCs) to mature oocytes. In this study, a systematic investigation of epigenetic modifications and DAZL gene expression during oogonia meiotic entry were performed. We found that the expression of DAZL was epigenetically regulated by DNA methylation of CpG islands within its promoter region. During meiotic entry, a continuously increasing level of 5hmC, a stable epigenetic marker usually associated with the activation of gene expression, was observed from 11.5 to 16.5 dpc (days post coitum). Meanwhile trimethylation of lysine 27 on histone3 (H3K27me3), usually associated with repression of gene expression, had a sustainable increase from 12.5 to 16.5 dpc. Finally, by equally dividing the ovaries into three regions representing the anterior, the middle, and the posterior of the ovary and performing immunofluorescence and qRT-PCR on the individual regions, we provided further evidences that the meiotic entry and progression of female germ cells is in an anterior to posterior pattern.