Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection
Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection
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DOI：
10.1016/j.vetmic.2007.08.034
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发表时间：
2008-04-01
影响因子：
3.3
通讯作者：
Aranaz, Alicia
中科院分区：
文献类型：
--
作者：
Alvarez, Julio;de Juan, Lucia;Aranaz, Alicia
检测受感染的动物是根除结核病计划的关键步骤。副结核感染已被证明损害了诊断试验的特异性。然而，其对其敏感性的影响尚未得到澄清。在本研究中，为了评估副结核对皮肤试验和干扰素- γ (ifn - γ)测定的敏感性可能的影响，我们对177只患有结核-副结核混合感染的山羊进行了皮肤试验和干扰素- γ测定。分枝杆菌培养作为金标准，以确定真实感染状态。所有技术的灵敏度都低于先前描述的；单次皮内结核菌素（SIT）试验和ifn - γ试验检出率为71% (62.4 ~ 78.6 95% C.I.)；单次皮内宫颈比较结核菌素（SICCT）试验仅检出42.7% （34.1-51.7,95% C.I.）的感染动物。当SIT试验和ifn - γ试验同时使用时，灵敏度最高（90.8%,84.5-95.2,95% C.I.）。还通过比较患有结核病的动物和患有混合感染的动物来评估测试的敏感性；研究发现，前一组的测试更有效。副结核病似乎对所研究的诊断试验的敏感性有重大影响，因此必须予以考虑；特别是，当结核和副结核同时存在时，应质疑SICCT检查的使用。(c) 2007 Elsevier B.V.版权所有
Detection of infected animals is a key step in eradication programs of tuberculosis. Paratuberculosis infection has been demonstrated to compromise the specificity of the diagnostic tests. However, its effect on their sensitivity has not been clarified. In the present study, skin tests and the interferon-gamma (IFN-gamma) assay were evaluated in a goat flock (n = 177) with a mixed tuberculosis-paratuberculosis infection in order to assess the possible effect of paratuberculosis on their sensitivity. Culture of mycobacteria was performed as the gold standard to determine the true infection status. All techniques showed lower sensitivities than previously described; the single intradermal tuberculin (SIT) test and the IFN-gamma assay detected 71% (62.4-78.6 95% C.I.) of the infected animals; the single intradermal cervical comparative tuberculin (SICCT) test detected only 42.7% (34.1-51.7, 95% C.I.) of infected animals. The highest level of sensitivity was obtained when SIT test and IFN-gamma assay were combined in parallel (90.8%, 84.5-95.2, 95% C.I.). Sensitivities of the tests were also assessed by comparing animals suffering tuberculosis and animals with a mixed infection; tests were found to be more effective in the former group. Paratuberculosis seems to have a major effect in the sensitivity of the diagnostic tests under study, and therefore must be taken into account; in particular, the use of the SICCT test should be questioned when both tuberculosis and paratuberculosis are present. (c) 2007 Elsevier B.V. All rights reserved.