Dependence of the acid-sensitive ion channel, ASIC1a, on extracellular Ca2+ ions
Dependence of the acid-sensitive ion channel, ASIC1a, on extracellular Ca2+ ions
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DOI：
10.1016/s0006-8993(01)02345-9
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发表时间：
2001-05-11
期刊：
影响因子：
2.9
通讯作者：
Bassilana, F
中科院分区：
文献类型：
--
作者：
de Weille, J;Bassilana, F
胚胎脊髓神经元的早期H+诱导电流依赖于细胞外Ca2+的功能。我们研究了在Cos细胞中表达的同源和异多聚酸敏感离子通道（asic）对Ca2+的依赖性。发现ASIC2a和ASIC1a通道的单通道电导在膜电位大于负-40 mV时因细胞外Ca2+升高而降低。由于这种对单一电流的影响，宏观ASIC2a在-60 mV时的峰值电流随着细胞外Ca2+浓度的升高而逐渐减小。此外，宏观ASIC1a电流在低细胞外Ca2+时非常小，并随着Ca2+的升高而增加，直至5 mM，然后在更高浓度的细胞外Ca2+下再次下降。(C) 2001 Elsevier Science B.V.版权所有
The early H+-induced current in the embryonic spinal cord neurone depends on extracellular Ca2+ for its function. We have studied the Ca2+-dependence of homo- and heteromultimeric acid-sensing ion channels (ASICs) expressed in Cos cells. It was found that single-channel conductance: of both the ASIC2a and the ASIC1a channel is reduced at membrane potentials more negative than -40 mV by elevated extracellular Ca2+. Due to this effect on unitary currents, the macroscopic ASIC2a peak current at -60 mV decreases gradually with rising extracellular Ca2+ concentration. In addition, the macroscopic ASIC1a current is very small at low extracellular Ca2+ and increases with rising Ca2+ up to 5 mM before decreasing again at still higher concentrations of extracellular Ca2+. (C) 2001 Elsevier Science B.V. All rights reserved.