CONJUGATION OF ANTIBODIES WITH BIFUNCTIONAL CHELATING-AGENTS - ISOTHIOCYANATE AND BROMOACETAMIDE REAGENTS, METHODS OF ANALYSIS, AND SUBSEQUENT ADDITION OF METAL-IONS
CONJUGATION OF ANTIBODIES WITH BIFUNCTIONAL CHELATING-AGENTS - ISOTHIOCYANATE AND BROMOACETAMIDE REAGENTS, METHODS OF ANALYSIS, AND SUBSEQUENT ADDITION OF METAL-IONS
复制标题
DOI：
10.1016/0003-2697(84)90517-7
复制
发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
MCTIGUE, M
中科院分区：
文献类型：
--
作者：
MEARES, CF;MCCALL, MJ;MCTIGUE, M
报道了螯合剂(S)-4-[2,3-双[双(羧甲基)氨基]丙基]苯基异硫氰酸酯的制备。描述了将其与 (S)-N-4-[2,3-双[双-(羧甲基)氨基]丙基]苯基溴乙酰胺与单克隆抗体和其他蛋白质缀合的程序。通过 Sephadex G-50 离心可以快速纯化缀合物。蛋白质结合的螯合基团的数量可以通过用标准 57Co2+ 滴定来测量，使用 TLC 来监测结合。标记的产品保留了其免疫反应性，正如用针对小鼠 I-AK 抗原的螯合物缀合抗体进行的体内实验所表明的那样。
Preparation of the chelating agent (S)-4-[2,3-bis[bis(carboxymethyl)amino]propyl]phenyl isothiocyanate is reported. Procedures for conjugation of this and (S)-N-4-[2,3-bis[bis-(carboxymethyl)amino]propyl]phenyl bromoacetamide to monoclonal antibodies and other proteins are described. The conjugates may be purified quickly by centrifugation through Sephadex G-50. The number of protein-bound chelating groups may be measured by titration with standard 57Co2+, using TLC to monitor binding. The labeled products retain their immunoreactivity, as illustrated by experiments in vivo with chelate-conjugated antibody to mouse I-AK antigen.