The influence of sample collection, handling and low temperature storage upon NMR metabolic profiling analysis in human synovial fluid
The influence of sample collection, handling and low temperature storage upon NMR metabolic profiling analysis in human synovial fluid
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DOI：
10.1016/j.jpba.2021.113942
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发表时间：
2021-02-16
影响因子：
3.4
通讯作者：
Gupte, Chinmay M.
中科院分区：
文献类型：
--
作者：
Jaggard, Matthew K. J.;Boulange, Claire L.;Gupte, Chinmay M.
代谢对关节疾病病理改变的影响正迅速被认为是一个重要的研究领域。关节滑液是关节疾病的一种有吸引力和代表性的生物流体。尽管最近的报告描述了关节疾病的代谢表型，但系统性综述显示，几乎没有证据表明滑膜关节液收集、处理或储存的代谢稳定性。我们的目的是报告在不同的储存温度，持续时间和条件下，使用核磁共振（NMR）光谱在人体滑液（HSF）中的小分子检测的变化。通过关节穿刺术从患有孤立性单关节病或接受关节置换术的患者（n = 30）中获得HSF。评估短期储存（0- 12小时，4摄氏度和18摄氏度）和重复冻融循环（-80摄氏度至18摄氏度）的效果。通过早期（-80 ℃，
The impact of metabolism upon the altered pathology of joint disease is rapidly becoming recognized as an important area of study. Synovial joint fluid is an attractive and representative biofluid of joint disease. A systemic review revealed little evidence of the metabolic stability of synovial joint fluid collection, handling or storage, despite recent reports characterizing the metabolic phenotype in joint disease. We aim to report the changes in small molecule detection within human synovial fluid (HSF) using nuclear magnetic resonance (NMR) spectroscopy at varying storage temperatures, durations and conditions. HSF was harvested by arthrocentesis from patients with isolated monoarthropathy or undergoing joint replacement (n = 30). Short-term storage (0-12h, 4 degrees C & 18 degrees C) and the effect of repeated freeze-thaw cycles (-80 degrees C to 18 degrees C) was assessed. Long-term storage was evaluated by early (-80 degrees C,