BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken
BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken
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DOI：
10.1002/dvdy.21510
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发表时间：
2008-04-01
影响因子：
2.5
通讯作者：
Sheng, Guojun
中科院分区：
文献类型：
--
作者：
Alev, Cantas;McIntyre, Brendan A. S.;Sheng, Guojun
红细胞中珠蛋白链的组成在发育和疾病过程中发生变化，反映了氧获取的生理变化和珠蛋白转录的调节变化。珠蛋白链组成与红细胞谱系/生态位之间的关系尚不清楚。通过结合蛋白质组学、基因组学和内含子原位杂交分析，研究人员发现，在鸡原始红细胞生成过程中，主要成年β -珠蛋白β - aa的转录本和蛋白产物早在主要胚胎β -珠蛋白出现时就存在了。从胚胎日(E) 5开始，β - A百分比迅速上升，到E7达到成人剖面。我们的数据表明，β - a基因座从原始红细胞生成开始就活跃，发育过程中的β -珠蛋白开关可能反映了相对转录物丰度的变化，而不是基因激活的严格开/关开关。
Reflecting physiological changes in oxygen acquisition and regulatory changes in globin transcription, the makeup of globin chains in erythrocytes varies in development and disease. The relationship between the globin chain composition and erythropoietic lineages/niches is not well-understood. Using a combination of proteomic-, genomic-, and intron-based in situ hybridization analyses, we show that the transcripts and protein product of the major adult beta globin, betaA, are present as early as the major embryonic beta globins during chicken primitive erythropoiesis. A rapid rise in betaA percentage is seen from embryonic day (E) 5, reaching adult profile by E7. Our data suggest that betaA locus is active from the onset of primitive erythropoiesis and that beta globin switching during development may reflect a change in relative transcript abundance rather than a strict on/off switch in gene activation.