Enzyme-free and label-free ultrasensitive electrochemical detection of DNA and adenosine triphosphate by dendritic DNA concatamer-based signal amplification
Enzyme-free and label-free ultrasensitive electrochemical detection of DNA and adenosine triphosphate by dendritic DNA concatamer-based signal amplification
复制标题
通过基于树状 DNA 串联体的信号放大对 DNA 和三磷酸腺苷进行无酶和无标记超灵敏电化学检测
DOI：
10.1016/j.bios.2013.12.066
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发表时间：
2014-06-15
影响因子：
12.6
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Liu, Shufeng;Lin, Ying;Li, Feng
杂交链反应（HCR）策略已经被很好地开发用于制造用于信号放大的各种生物传感平台。本文首次提出了一种新型的无酶、无标记的超灵敏电化学DNA生物传感平台，用于检测目标DNA和三磷酸腺苷（ATP），其中巧妙设计了三个辅助DNA探针，通过HCR策略构建了树枝状DNA多联体，并作为六氯化氨合钌（RuHex）载体进行信号放大。该DNA生物传感器的信号放大策略可实现对DNA和ATP的超灵敏电化学检测，其上级检测限分别低至5 aM和20 fM，同时对DNA和ATP的检测也表现出很高的选择性。目前提出的树突状DNA多联体为构建超灵敏的DNA生物传感平台开辟了一个很有前途的方向，用于生物分析和临床生物医学中的生物分子检测，由于不需要任何类型的酶，化学修饰或标记，因此具有简单和成本效益的明显优势。(C)2014爱思唯尔有限公司版权所有。
Hybridization chain reaction (HCR) strategy has been well developed for the fabrication of various biosensing platforms for signal amplification. Herein, a novel enzyme-free and label-free ultrasensitive electrochemical DNA biosensing platform for the detection of target DNA and adenosine triphosphate (ATP) was firstly proposed, in which three auxiliary DNA probes were ingeniously designed to construct the dendritic DNA concatamer via HCR strategy and used as hexaammineruthenium(III) chloride (RuHex) carrier for signal amplification. With the developed dendritic DNA concatamer-based signal amplification strategy, the DNA biosensor could achieve an ultrasensitive electrochemical detection of DNA and ATP with a superior detection limit as low as 5 aM and 20 fM, respectively, and also demonstrate a high selectivity for DNA and ATP detection. The currently proposed dendritic DNA concatamer opens a promising direction to construct ultrasensitive DNA biosensing platform for biomolecular detection in bioanalysis and clinical biomedicine, which offers the distinct advantages of simplicity and cost efficiency owing to no need of any kind of enzyme, chemical modification or labeling. (C) 2014 Elsevier B.V. All rights reserved.