Aggregation of dipalmitoylphosphatidylcholine vesicles.
Aggregation of dipalmitoylphosphatidylcholine vesicles.
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DOI：
10.1021/bi00260a033
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发表时间：
1982-08
期刊：
影响因子：
2.9
通讯作者：
M. Wong;T. E. Thompson
中科院分区：
文献类型：
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作者：
M. Wong;T. E. Thompson
准弹性和90度光散射被用来研究聚集的二棕榈酰磷脂酰胆碱囊泡在温度低于凝胶液晶相变作为浓度，温度和大小的函数。囊泡浓度的增加并没有明显改变聚集体的大小，但确实改变了聚集体的总数，其方式与聚集体向融合囊泡转化的双分子碰撞机制一致。增加温度降低聚集，表明解聚速率常数增加快于聚集速率常数。作为大小的函数，聚集从小到700 A直径囊泡略微降低，并且对于950 A直径囊泡显著增加。一个模型的相互作用的小泡下面的凝胶液晶相转变，其中聚集之前的融合和聚集体之间的碰撞触发融合。
Quasi-elastic and 90 degrees light scattering were used to study the aggregation of dipalmitoylphosphatidylcholine vesicles at temperatures below the gel-liquid-crystalline phase transition as a function of concentration, temperature, and size. Increased vesicle concentration did not appreciably change aggregate size but did change the total number of aggregates in a manner consistent with a bimolecular collisional mechanism for the conversion of aggregates to fused vesicles. Increased temperature decreased aggregation, indicating that the disaggregation rate constant increased faster than the aggregation rate constant. As a function of size, aggregation decreased slightly from small to 700 A diameter vesicles and increased considerably for 950 A diameter vesicles. A model of the interaction of small vesicles below the gel-liquid-crystalline phase transition is presented in which aggregation precedes fusion and collision between aggregates triggers fusion.