Pharmacological Transection of Brain-Spinal Cord Communication Blocks Pain-Induced Hemorrhage and Locomotor Deficits after Spinal Cord Injury in Rats
Pharmacological Transection of Brain-Spinal Cord Communication Blocks Pain-Induced Hemorrhage and Locomotor Deficits after Spinal Cord Injury in Rats
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DOI：
10.1089/neu.2019.6973
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发表时间：
2020-08-01
影响因子：
4.2
通讯作者：
Grau, James W.
中科院分区：
文献类型：
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作者：
Davis, Jacob A.;Bopp, Anne C.;Grau, James W.
脊髓损伤 (SCI) 通常伴有额外的组织损伤（多发伤），这会涉及疼痛（伤害性）纤维。先前的研究表明，伤害性输入会增加细胞死亡，扩大出血面积，并损害长期恢复。目前的研究表明，这些不良反应可以通过在挫伤处涂抹钠通道阻滞剂利多卡因来阻止。大鼠受到下胸椎 (T12) 挫伤，24 小时后对尾部施加有害电刺激（电击）。在休克治疗之前，通过在 T2 缓慢输注利多卡因来进行药理横断。在接下来的 21 天内评估长期运动恢复情况。有害的电刺激会损害运动恢复，而头端利多卡因会阻断这种作用。接下来，评估利多卡因的急性作用。有害刺激后 3 小时收集组织，并通过使用蛋白质印迹法评估血红蛋白含量来评估出血程度。伤害性刺激增加了出血程度。利多卡因在 T2 之前施用，但不是在之后立即施用，刺激阻断了这种效应。当通过腰椎穿刺将利多卡因应用于受伤部位时，观察到类似的结果。结果表明，药理横断可以阻止伤害感受引起的出血和运动缺陷的加剧。
Spinal cord injury (SCI) is often accompanied by additional tissue damage (polytrauma), which engages pain (nociceptive) fibers. Prior research has shown that nociceptive input can increase cell death, expand the area of hemorrhage, and impair long-term recovery. The current study shows that these adverse effects can be blocked by the sodium channel blocker lidocaine applied rostral to a contusion injury. Rats received a lower thoracic (T12) contusion injury, and noxious electrical stimulation (shock) was applied to the tail 24 h later. Immediately before shock treatment, a pharmacological transection was performed by slowly infusing lidocaine at T2. Long-term locomotor recovery was assessed over the next 21 days. Noxious electrical stimulation impaired locomotor recovery, and this effect was blocked by rostral lidocaine. Next, the acute effect of lidocaine was assessed. Tissue was collected 3 h after noxious stimulation, and the extent of hemorrhage was evaluated by assessing hemoglobin content using Western blotting. Nociceptive stimulation increased the extent of hemorrhage. Lidocaine applied at T2 before, but not immediately after, stimulation blocked this effect. A similar pattern of results was observed when lidocaine was applied at the site of injury by means of a lumbar puncture. The results show that a pharmacological transection blocks nociception-induced hemorrhage and exacerbation of locomotor deficits.