Infrared and Raman Spectroscopic Studies of Molecular Disorders in Skin Cancer
Infrared and Raman Spectroscopic Studies of Molecular Disorders in Skin Cancer
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DOI：
10.21873/invivo.11512
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发表时间：
2019-03-01
期刊：
影响因子：
2.3
通讯作者：
Theophanides, Theophile
中科院分区：
文献类型：
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作者：
Anastassopoulou, Jane;Kyriakidou, Maria;Theophanides, Theophile
目的：探讨癌变皮肤的分子结构紊乱。材料与方法：采用人恶性黑色素瘤和基底细胞癌活检进行研究。利用傅里叶变换红外（FT-IR），拉曼光谱和扫描电子显微镜。记录健康、基底细胞癌和黑色素瘤组织的光谱差异。结果：黑色素瘤中细胞膜脂质v(as)CH(2)、v(s)CH(2)和Raman v(s)CH(3)的FT-IR波段由于亲脂性环境的增加而增强。在1744 cm（-1）处分配给丙二醛的FT-IR波段可以用作癌症进展的波段诊断。Raman和FT-IR分别在1654 cm（-1）和1650 cm（-1）处的酰胺I波段在黑色素瘤中较宽，反映了蛋白质二级结构从α -螺旋到β -片和随机线圈的变化。在1046 cm（-1）处的FT-IR波段强度在黑色素瘤中增加，表明在癌症发展过程中皮肤糖基化。另一个可能被认为具有诊断意义的条带位于黑色素瘤约815 cm（-1）处，归因于Z-DNA结构。据我们所知，这是第一次通过扫描电子显微镜发现，从牙齿植入物中提取的钛合金金属成分被转移到一名患者背部的黑色素瘤组织中。结论：振动光谱显示黑色素瘤中糖基化增加。
Aim: To investigate the molecular structural disorders of cancerous skin. Materials and Methods: Human malignant melanoma and basal cell carcinoma biopsies were used for the investigation. Fourier transform infrared (FT-IR), Raman spectroscopy, and scanning electron microscopy were utilized. Spectral differences between healthy, basal cell carcinoma and melanoma tissues were recorded. Results: The FT-IR bands of v(as)CH(2), v(s)CH(2) and Raman v(s)CH(3) of cell membrane lipids were increased in intensity in melanoma due to an increased lipophilic environment. The FT-IR band at 1,744 cm(-1) assigned to malondialdehyde can be used as a band diagnostic of cancer progression. The amide I bands at 1,654 cm(-1) and 1,650 cm(-1) for Raman and FT-IR, respectively were broader in spectra from melanoma, reflecting changes of protein secondary structure from alpha-helix to beta-sheet and random coil. The intensity of the FT-IR band at 1,046 cm(-1) was increased in melanoma, suggesting glycosylation of the skin upon cancer development. Another band that might be considered as diagnostic was found at about 815 cm(-1) in melanoma and was attributed to Z-DNA configuration. As far as we know, this is the first time that scanning electron microscopy revealed that metal components of titanium alloys from tooth implants were transferred to melanoma tissue taken from the back of one patient. Conclusion: Vibrational spectroscopy highlighted increased glycosylation in melanoma.