High-level iodination of monoclonal antibody fragments for radiotherapy.
High-level iodination of monoclonal antibody fragments for radiotherapy.
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发表时间：
1984-03
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通讯作者：
J. Ferens;K. Krohn;P. Beaumier;J. Brown;I. Hellström;K. Hellström;J. Carrasquillo;S. Larson
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作者：
J. Ferens;K. Krohn;P. Beaumier;J. Brown;I. Hellström;K. Hellström;J. Carrasquillo;S. Larson
两个不同的针对黑色素瘤相关抗原p97的鼠单抗Fab片段被I-131以高活性水平标记，而没有过多的化学损伤。使用氯胺-T方法和在室温下的大工作体积，用高达300 mCI的I-131标记多达20毫克的FAB。高达90%的初始活性以标记产物的形式被回收。通过体外细胞结合试验，标记的纤维对放射性碘损伤的敏感性不同。使用远程碘化仪安全地进行放射性碘化。最终产品具有放射性药物性质，适用于临床诊断和人体实验放射治疗。
Two different murine monoclonal antibody Fab fragments specific for p97, a melanoma-associated antigen, were labeled with I-131 at high activity levels without excessive chemical damage. Up to 20 mg of Fab were labeled with up to 300 mCi of I-131 using the chloramine-T method and large working volumes at room temperature. As much as 90% of the initial activity was recovered as labeled product. The labeled Fabs varied in their sensitivity to radioiodination damage, as measured by an in vitro cell-binding assay. Radioiodination was performed safely using a remote iodination apparatus. The final product was of radiopharmaceutical quality suitable for clinical diagnosis and experimental radiotherapy in humans.