Chitosan and Sodium Alginate Combinations Are Alternative, Efficient, and Safe Natural Adjuvant Systems for Hepatitis B Vaccine in Mouse Model.
Chitosan and Sodium Alginate Combinations Are Alternative, Efficient, and Safe Natural Adjuvant Systems for Hepatitis B Vaccine in Mouse Model.
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DOI：
10.1155/2016/7659684
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Amin MA
中科院分区：
文献类型：
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作者：
AbdelAllah NH;Abdeltawab NF;Boseila AA;Amin MA
B型肝炎病毒（HBV）感染是一个重大的公共卫生问题，也是医护人员的职业危害。目前以明矾为佐剂的乙肝疫苗是预防乙肝病毒感染的最有效措施。然而，该疫苗具有一些局限性，包括在一些疫苗接种者中的应答差以及是霜敏感的悬浮液。我们研究的目的是使用一种替代的天然佐剂系统，其免疫原性强，可以减少剂量和成本。我们在小鼠模型中测试了用壳聚糖（Ch）和海藻酸钠（S）作为佐剂的HBV表面抗原（HBsAg），这两种天然佐剂单独或与明矾组合。小鼠组皮下免疫HBsAg佐剂与Ch或S，或三佐剂配方与明矾（Al），Ch，和S，或双重制剂与AlCh或AlS。将这些与用当前疫苗配方或无佐剂HBsAg免疫的对照组进行比较。我们评估了血清转换率、血清HBsAg抗体、IL-4和IFN-γ水平。结果表明，含有Ch或S的溶液配方对Al佐剂悬浮液表现出相当的免疫原性应答。与对照相比，AlChS产生了显著更高的免疫原性应答。总的来说，我们的研究结果表明，Ch和S是有效的HBV佐剂，提供了天然的替代品，有可能减少剂量。
Hepatitis B viral (HBV) infections represent major public health problem and are an occupational hazard for healthcare workers. Current alum-adjuvanted HBV vaccine is the most effective measure to prevent HBV infection. However, the vaccine has some limitations including poor response in some vaccinee and being a frost-sensitive suspension. The goal of our study was to use an alternative natural adjuvant system strongly immunogenic allowing for a reduction in dose and cost. We tested HBV surface antigen (HBsAg) adjuvanted with chitosan (Ch) and sodium alginate (S), both natural adjuvants, either alone or combined with alum in mouse model. Mice groups were immunized subcutaneously with HBsAg adjuvanted with Ch or S, or triple adjuvant formula with alum (Al), Ch, and S, or double formulations with AlCh or AlS. These were compared to control groups immunized with current vaccine formula or unadjuvanted HBsAg. We evaluated the rate of seroconversion, serum HBsAg antibody, IL-4, and IFN-γ levels. The results showed that the solution formula with Ch or S exhibited comparable immunogenic responses to Al-adjuvanted suspension. The AlChS gave significantly higher immunogenic response compared to controls. Collectively, our results indicated that Ch and S are effective HBV adjuvants offering natural alternatives, potentially reducing dose.