Renal arterial 20-hydroxyeicosatetraenoic acid levels: regulation by cyclooxygenase
Renal arterial 20-hydroxyeicosatetraenoic acid levels: regulation by cyclooxygenase
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DOI：
10.1152/ajprenal.00239.2002
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发表时间：
2003-03-01
影响因子：
4.2
通讯作者：
Carroll, MA
中科院分区：
文献类型：
--
作者：
Cheng, MK;McGiff, JC;Carroll, MA
20-HETE, a potent vasoconstrictor, is generated by cytochrome P-450 omega-hydroxylases and is the principal eicosanoid produced by preglomerular microvessels. It is released from preglomerular microvessels by ANG II and is subject to metabolism by cyclooxygenase (COX). Because low-salt (LS) intake stimulates the renin-angiotensin system and induces renal cortical COX-2 expression, we examined 20-HETE release from renal arteries (interlobar and arcuate and interlobular arteries) obtained from 6- to 7-wk-old male Sprague-Dawley rats fed either normal salt (0.4% NaCl) or LS (0.05% NaCl) diets for 10 days. With normal salt intake, the levels of 20-HETE recovered were similar in arcuate and interlobular arteries and interlobar arteries: 30.1+/-8.5 vs. 24.6+/-5.3 ng.mg protein(-1).30min(-1), respectively. An LS diet increased 20-HETE levels in the incubate of either arcuate and interlobular or interlobar renal arteries only when COX was inhibited. Addition of indomethacin (10 muM) to the incubate of arteries obtained from rats fed an LS diet resulted in a two- to threefold increase in 20-HETE release from arcuate and interlobular arteries, from 39.1+/-13.2 to 101.8+/-42.6 ng.mg protein-(1).30 min(-1) (P
