Loss of regulation of lipogenesis in the Zucker diabetic (ZDF) rat
Loss of regulation of lipogenesis in the Zucker diabetic (ZDF) rat
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DOI：
10.1152/ajpendo.2000.279.2.e425
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发表时间：
2000-08-01
影响因子：
5.1
通讯作者：
Boros, LG
中科院分区：
文献类型：
--
作者：
Lee, WNP;Bassilian, S;Boros, LG
我们在这里提出了一个研究的作用，瘦素在脂肪生成的调节，通过检查饮食中的大量营养素组成对脂肪生成的瘦素受体缺陷的Zucker糖尿病肥胖大鼠（ZDF）和它的瘦窝（ZL）的影响。动物成对喂食两种等热量饮食，其脂肪与碳水化合物的比例不同，提供10%和30%的能量作为脂肪。使用氘代水和同位素分析测量大鼠中的脂肪生成。从氘掺入到血浆棕榈酸酯，硬脂酸酯，油酸酯，我们确定从头合成棕榈酸酯和合成硬脂酸酯的链延长和油酸酯的去饱和。由于大量营养素的组成和热量密度的控制，在这些饮食条件下的从头脂肪生成的变化表示适应饮食中的脂肪-碳水化合物比例的变化。在ZL大鼠中，高脂饮食通常会抑制新生脂肪生成，以维持相对恒定的脂质转运量。ZDF大鼠具有较高的脂肪生成率，这并不受高脂饮食的抑制。结果表明，瘦素在脂肪生成的反馈调节中具有重要的激素作用。
We present here a study on the role of leptin in the regulation of lipogenesis by examining the effect of dietary macronutrient composition on lipogenesis in the leptin receptor-defective Zucker diabetic fatty rat (ZDF) and its lean litter mate (ZL). Animals were pair fed two isocaloric diets differing in their fat-to-carbohydrate ratio providing 10 and 30% energy as fat. Lipogenesis was measured in the rats using deuterated water and isotopomer analysis. From the deuterium incorporation into plasma palmitate, stearate, and oleate, we determined de novo synthesis of palmitate and synthesis of stearate by chain elongation and of oleate by desaturation. Because the macronutrient composition and the caloric density were controlled, changes in de novo lipogenesis under these dietary conditions represent adaptation to changes in the fat-to-carbohydrate ratio of the diet. De novo lipogenesis was normally suppressed in response to the high-fat diet in the ZL rat to maintain a relatively constant amount of lipids transported. The ZDF rat had a higher rate of lipogenesis, which was not suppressed by the high-fat diet. The results suggest an important hormonal role of leptin in the feedback regulation of lipogenesis.