Aqueous Flow Measured by Fluorophotometry in the Mouse.
Aqueous Flow Measured by Fluorophotometry in the Mouse.
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DOI：
10.1167/iovs.14-15144
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发表时间：
2016-07-01
影响因子：
4.4
通讯作者：
Ishimoto BM
中科院分区：
文献类型：
--
作者：
Toris CB;Fan S;Johnson TV;Camras LJ;Hays CL;Liu H;Ishimoto BM
设计用来测量小鼠眼睛房水流量的荧光光度计在人工荧光素小室中进行了测试，并在不同麻醉方案、房水流量抑制剂和前房插管法的活体小鼠身上进行了测试。在局部应用荧光素两小时后，一组CD-1小鼠被氯胺酮/赛拉津、2，2，2-三溴乙醇或氯胺酮单独麻醉。用荧光光度扫描仪定期测量角膜和前房荧光素浓度60~90分钟，以计算房水流量。随后，一组小鼠接受了前房插管测量房水流量。第三组在荧光扫描前1小时以交叉方式接受噻吗洛尔、多唑胺和赋形剂治疗。氯胺酮/赛拉津复合麻醉时房水流量(0.09±0.05min0.05min0.05min0.05min0.05min0.05min.L，平均±SD，n=24)明显慢于三溴乙醇或氯胺酮(P<；0.001)。在三溴乙醇麻醉下，噻吗洛尔使房水流量由(0.20±0.07)μL/分钟降至(0.07±0.03)μL/分钟(P=0.001)；在氯胺酮麻醉下，眼房水流量由(0.14±0.03)μL/分钟降至(0.10±0.02)μL/分钟(P=0.004)。多唑胺使房水流量由0.09±0.03降至0.06±0.03μL/min(P=0.04)。前房插管法的房水流量(0.2 0±0.13μ)明显高于荧光法(0.0 9±0.0 7μL)(P=0.0 5)。一种新的非侵入性荧光光度法检测了全身麻醉和已知的含水抑制剂对小鼠房水流动的影响。荧光分光光度法测得的水流量比插管法慢，而且在技术上更容易，变异性更小。小鼠荧光分光光度计可用于重复测量小鼠眼内房水的流动，使交叉和纵向研究成为可能。
A fluorophotometer designed to measure aqueous flow in murine eyes was tested with artificial fluorescein chambers and in live mice with different anesthesia regimens, aqueous flow suppressants, and an anterior chamber cannulation method. Two hours following topical fluorescein application, one group of CD-1 mice was anesthetized with ketamine/xylazine, 2,2,2-tribromoethanol, or ketamine alone. Cornea and anterior chamber fluorescein concentrations were measured periodically for 60 to 90 minutes by fluorophotometric scans to calculate aqueous flow. Later, a subgroup of mice underwent aqueous flow measurement by anterior chamber cannulation. A third group was treated with timolol, dorzolamide, and vehicle in a crossover manner 1 hour prior to fluorophotometric scans. Aqueous flow with ketamine/xylazine anesthesia (0.09 ± 0.05 μL/min, mean ± SD, n = 24) was slower than with tribromoethanol or ketamine alone (P < 0.001). Timolol reduced aqueous flow from 0.20 ± 0.07 μL/min to 0.07 ± 0.03 μL/min (P = 0.001) under tribromoethanol anesthesia and from 0.14 ± 0.03 μL/min to 0.10 ± 0.02 μL/min (P = 0.004) under ketamine anesthesia but not under ketamine/xylazine anesthesia. Dorzolamide reduced aqueous flow from 0.09 ± 0.03 to 0.06 ± 0.03 μL/min (P = 0.04) under ketamine/xylazine anesthesia. Aqueous flow by anterior chamber cannulation (0.20 ± 0.13 μL/min) was greater (P = 0.05) than by fluorophotometry (0.09 ± 0.07 μL/min). A new noninvasive fluorophotometric method detected effects of general anesthesia and known aqueous suppressants on aqueous flow in mice. Aqueous flow measured by fluorophotometry was slower than by cannulation, and was technically easier with less variability. The mouse fluorophotometer is useful for repeated measurements of aqueous flow in the murine eye making crossover and longitudinal studies possible.