IMAGING OF THIN-LAYER CHROMATOGRAMS USING MATRIX/ASSISTED LASER DESORPTION/IONIZATION MASS-SPECTROMETRY
IMAGING OF THIN-LAYER CHROMATOGRAMS USING MATRIX/ASSISTED LASER DESORPTION/IONIZATION MASS-SPECTROMETRY
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DOI：
10.1021/ac00120a021
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发表时间：
1995-12-15
影响因子：
7.4
通讯作者：
HERCULES, DM
中科院分区：
文献类型：
--
作者：
GUSEV, AI;VASSEUR, OJ;HERCULES, DM
介绍了一种将薄层色谱与基质辅助激光解吸电离质谱仪直接联用的新方法。为了避免分析物在薄层色谱板上过度扩散，首先在不同的底物上生成基质层，从该基质层转移到板上。转移是通过将基质压到先前湿润的TLC板上完成的。对于罗丹明B、几内亚绿B、缓激肽和血管紧张素II等几种化合物，用纳克量的分析物成功地直接成像了高空间分辨率(250-500微米)和高灵敏度的硅胶薄层，由于基质的不均一性和产生良好信号强度所需的最小激光光束直径，最终的空间分辨率估计接近50微米。目前的方法产生了最小的分析物平面扩散(类似于0.5 mm)，并导致了在皮克范围内的绝对检测限。
A new approach for directly coupling thin-layer chromatography (TLC) with matrix-assisted laser desorption/ ionization mass spectrometry is introduced. To avoid excessive analyte spreading along a TLC plate, a matrix layer was first generated on a different substrate, from which it was transferred to the plate. The transfer was accomplished by pressing the matrix onto a previously wetted TLC plate. Successful direct imaging of silica gel TLC plates with high spatial resolution (250-500 mu m) and high sensitivity has been demonstrated for several compounds, including rhodamine B, guinea green B, bradykinin, and angiotensin II, using nanogram quantities of analyte, The ultimate spatial resolution was estimated to be similar to 50 mu m due to matrix heterogeneity and the minimum laser beam diameter required to produce good signal intensity. The current protocol induced minimal analyte planar diffusion (similar to 0.5 mm) and resulted in an absolute detection limit in the picogram range.