Neovascularization of osteoporotic metaphyseal bone defects: A morphometric micro-CT study
Neovascularization of osteoporotic metaphyseal bone defects: A morphometric micro-CT study
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DOI：
10.1016/j.mvr.2015.10.005
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发表时间：
2016-05-01
影响因子：
3.1
通讯作者：
Langheinrich, Alexander C.
中科院分区：
文献类型：
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作者：
Kampschulte, Marian;Krombach, Gabriele A.;Langheinrich, Alexander C.
目的：新生血管是骨折骨再生的关键。本研究旨在探讨微血管的形态和分布在非受伤的股骨和新生血管的干骺端临界尺寸缺损的小动物模型osteoporosis.Materials和Methods：雌性大鼠（n = 7）卵巢切除（OVX），并收到了multideficiency饮食。OVX后3个月，在股骨远端干骺端切割一个5 mm楔形临界尺寸缺损，并用T形微型钢板固定。6周后处死动物，取股骨脱钙，用显微CT测量骨折新生血管形成情况。结果：骨折沿着临界尺寸缺损未见愈合。在未受伤的骨，微血管分布显示出特定的模式，从而使表皮，Meta和骨干之间的分化。基于显微CT的形态测量显示，与完整的对侧股骨相比，临界尺寸缺损的血管体积分数和血管厚度显著降低（p < 0.001）。与血容量相关的血管表面（血管表面/体积）显著增加（p < 0.001）。连接密度和组织体积相关的血管surface.Conclusions血管表面密度没有显着变化：Micro-CT血管形态测量显示epi，Meta和骨干之间的差异，在非受伤的骨，以及之间的差异的临界大小的缺陷和非受伤的干骺端。由于血管生成是骨生成之前的一个重要先决条件，我们的研究结果可能会影响在这种骨质疏松症的小动物模型中对骨传导或成骨生物材料的进一步评价。(C)2015 Elsevier Inc. All rights reserved.
Purpose: Neovascularization is essential for bone regeneration in fractures. This study aimed to investigate the microvascular morphology and distribution in the non-injured femur and the neovascularization of the metaphyseal critical size defect in a small animal model of osteoporosis.Materials and methods: Female rats (n = 7) were ovariectomized (OVX) and received a multideficiency diet. Three months after OVX, a 5 mm wedge shaped critical size defect was cut at the distal femoral metaphysis and stabilized with a T-shaped mini-plate. After six weeks, the animals were euthanized, and femora were removed and decalcified for micro-CT measurement of fracture neovascularization.Results: No fracture healing was observed along the critical size defects. In the non-injured bone, micro-vessel distribution showed a specific pattern, thereby enabling a differentiation between epi-, meta- and diaphysis. Micro-CT based morphometry revealed a significant reduction of the vascular volume fraction as well as the vascular thickness (p < 0.001) in the critical size defect compared to the intact contralateral femur. Blood volume related vascular surface (vascular surface/volume) increased significantly (p < 0.001). Connectivity density and tissue volume related vascular surface (vascular surface density) did not change significantly.Conclusions: Micro-CT based vascular morphometry demonstrated differences between epi-, meta- and diaphysis in the non-injured bone as well as differences between the critical size defect and the non-injured metaphysis. As angiogenesis is a crucial prerequisite that precedes osteogenesis, our results may influence further evaluation of osteoconductive or osteogenic biomaterials in this small animal model of osteoporosis. (C) 2015 Elsevier Inc. All rights reserved.