Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome
Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome
复制标题
DOI：
10.1007/s00122-006-0447-3
复制
发表时间：
2007-02-01
影响因子：
5.4
通讯作者：
Abad, P.
中科院分区：
文献类型：
--
作者：
Djian-Caporalino, C.;Fazari, A.;Abad, P.
根结线虫（Meloidogyne spp.）是一种主要的植物病原体，影响世界上几种茄类作物。辣椒对该病菌的抗性是由几个独立的显性基因控制的。六个Me基因先前在三个高抗性和遗传距离较远的材料中被证明在高温下稳定：PI 322719， PI 201234和CM334 （Criollo de Morelos 334）。一些基因（Me4、Me1和mec2）对某些棉球线虫物种或种群是特异性的，而另一些基因（Me1、Me3和Me7）对范围广泛的棉球线虫物种有效，包括地中海和热带地区最常见的棉球线虫，如沙场棉球线虫、爪哇棉球线虫和不知名棉球线虫。这些基因在根细胞中指导不同的反应模式，取决于辣椒系和线虫种类。等位基因测试和使用BSA-AFLP方法的精细定位表明，这些基因不同但有联系，重组频率为0.02-0.18。在几个遗传背景中鉴定出的基于pcr的标记中有三个与六个Me基因共同。CarthaGene软件比对表明，这6个基因在28 cM的间隔内聚集在一个基因组区域。在辣椒种内参考图谱上，用4个标记将该簇固定在P9染色体上。早先已在该群集附近绘制了其他抗病因素图。该基因组区与番茄的T12染色体和马铃薯的XII染色体共线。此前在该地区还发现了另外4个线虫抗性基因，表明这些线虫抗性基因位于茄科植物的同源基因组区域。
The root-knot nematode (Meloidogyne spp.) is a major plant pathogen, affecting several solanaceous crops worldwide. In Capsicum annuum, resistance to this pathogen is controlled by several independent dominant genes-the Me genes. Six Me genes have previously been shown to be stable at high temperature in three highly resistant and genetically distant accessions: PI 322719, PI 201234, and CM334 (Criollo de Morelos 334). Some genes (Me4, Mech1, and Mech2) are specific to certain Meloidogyne species or populations, whereas others (Me1, Me3, and Me7) are effective against a wide range of Meloidogyne species, including M. arenaria, M. javanica, and M. incognita, the most common species in Mediterranean and tropical areas. These genes direct different response patterns in root cells depending on the pepper line and nematode species. Allelism tests and fine mapping using the BSA-AFLP approach showed these genes to be different but linked, with a recombination frequency of 0.02-0.18. Three of the PCR-based markers identified in several genetic backgrounds were common to the six Me genes. Comparative mapping with CarthaGene software indicated that these six genes clustered in a single genomic region within a 28 cM interval. Four markers were used to anchor this cluster on the P9 chromosome on an intraspecific reference map for peppers. Other disease resistance factors have earlier been mapped in the vicinity of this cluster. This genomic area is colinear to chromosome T12 of tomato and chromosome XII of potato. Four other nematode resistance genes have earlier been identified in this area, suggesting that these nematode resistance genes are located in orthologous genomic regions in Solanaceae.