The Encystment-Related MicroRNAs and Its Regulation Molecular Mechanism in Pseudourostyla cristata Revealed by High Throughput Small RNA Sequencing
The Encystment-Related MicroRNAs and Its Regulation Molecular Mechanism in Pseudourostyla cristata Revealed by High Throughput Small RNA Sequencing
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高通量小RNA测序揭示拟尾柱花成膜相关MicroRNA及其调控分子机制
DOI：
10.3390/ijms21072309
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发表时间：
2020-04-01
影响因子：
5.6
通讯作者：
Chen, Jiwu
中科院分区：
文献类型：
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作者：
Pan, Nan;Bhatti, Muhammad Zeeshan;Chen, Jiwu
MicroRNA（miRNA）在多种细胞过程中调节靶基因的表达，并在不同的生理过程中发挥重要作用。然而，人们对纤毛原生动物包囊过程中的微小RNA组（miRNAome）知之甚少。在本研究中，我们首先研究了拟尾柱嵴（P. cristata）营养细胞转化为休眠包囊过程中差异表达的miRNA和相关信号通路。两个文库共发现 1608 个已知 miRNA。有 165 个 miRNA 和 1217 个目标 miRNA。在营养细胞和休眠包囊数据库之间筛选的差异 miRNA 总数为 449 个，p < 0.05 且 |log2 倍数变化| > 1.其中上调和下调的miRNA分别为243个和206个。此外，京都基因与基因组百科全书（KEGG）分析显示，一些差异表达的miRNA主要与氧化磷酸化、二组分系统和氨基酸的生物合成有关。结合我们的生物信息学分析，一些差异表达的 miRNA 包括 miR-143、miR-23b-3p、miR-28 和 miR-744-5p 参与了 P. cristata 的包囊。基于这些发现，我们提出了一个假设的调节或促进 P. cristata 包囊的 miRNA 信号网络。这项研究为纤毛原生动物包囊中 miRNA 的调控机制提供了新的线索。
MicroRNAs (miRNAs) regulate the expression of target genes in diverse cellular processes and play important roles in different physiological processes. However, little is known about the microRNAome (miRNAome) during encystment of ciliated protozoa. In the current study, we first investigated the differentially expressed miRNAs and relative signaling pathways participating in the transformation of vegetative cells into dormant cysts of Pseudourostyla cristata (P. cristata). A total of 1608 known miRNAs were found in the two libraries. There were 165 miRNAs with 1217 target miRNAs. The total number of differential miRNAs screened between vegetative cells and dormant cysts databases were 449 with p < 0.05 and |log2 fold changes| > 1. Among them, the upregulated and downregulated miRNAs were 243 and 206, respectively. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that some of the differentially expressed miRNAs were mainly associated with oxidative phosphorylation, two-component system, and biosynthesis of amino acids. Combining with our bioinformatics analyzes, some differentially expressed miRNAs including miR-143, miR-23b-3p, miR-28, and miR-744-5p participates in the encystment of P. cristata. Based on these findings, we propose a hypothetical signaling network of miRNAs regulating or promoting P. cristata encystment. This study shed new lights on the regulatory mechanisms of miRNAs in encystment of ciliated protozoa.