A novel micro-straw for cryopreservation of small number of human spermatozoon.
A novel micro-straw for cryopreservation of small number of human spermatozoon.
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一种用于冷冻保存少量人类精子的新型微型吸管
DOI：
10.4103/1008-682x.173452
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发表时间：
2017-05
影响因子：
2.9
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Liu F;Zou SS;Zhu Y;Sun C;Liu YF;Wang SS;Shi WB;Zhu JJ;Huang YH;Li Z
少数精子的冷冻保存仍然是男性生育能力保存的一大挑战。本研究报告了一种新型的微吸管(LSL吸管)用于冷冻少数精子进行卵胞浆内单精子注射(ICSI)。采集22例有生育能力的捐精者精液，每份精液标本稀释后与冷冻保护剂按1：1比例混合，分别用LSL吸管(50-100μL)、传统0.25ml和0.5ml三种不同的吸管进行冷冻。冷冻前、后用液氮熏蒸，直接降温至−130~−140°C，观察冷冻前后精子密度、精子前向活动率、精子形态、顶体完整性和DNA断裂指数。冷冻解冻后，LSL稻草组的精子活率显著高于传统的0.25ml和0.5ml稻草组(38.5%vs27.4%和25.6%，P<；0.003)。冷冻-解冻后精子活力和顶体完整率均显著低于冷冻前。然而，三种类型的吸管在形态、顶体和DNA完整性方面没有显著差异(P>；0.05)。由于LSL秸秆较薄，体积很小，所以LSL秸秆的冷冻速度明显快于0.25ml秸秆和0.5ml秸秆。综上所述，对于ICSI患者，LSL微吸管可以保存较少的运动精子，且运动能力恢复良好。
Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw (LSL straw) for freezing few spermatozoa for intracytoplasmic sperm injection (ICSI). Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1:1, and then frozen using three different straws such as LSL straw (50-100 μl), traditional 0.25 ml and 0.5 ml straws. For freezing, all straws were fumigated with liquid nitrogen, with temperature directly reducing to −130-−140°C. Sperm concentration, progressive motility, morphology, acrosome integrity, and DNA fragmentation index were evaluated before and after freezing. After freezing-thawing, LSL straw group had significantly higher percentage of sperm motility than traditional 0.25 ml and 0.5 ml straw groups (38.5% vs 27.4% and 25.6%, P < 0.003). Sperm motility and acrosomal integrity after freezing-thawing were significantly lower than that of before freezing. However, there was no significant difference in morphology, acrosome, and DNA integrity between the three types of straws (P > 0.05). As LSL straws were thinner and hold very small volume, the freezing rate of LSL straw was obviously faster than 0.25 ml straw and 0.5 ml straws. In conclusion, LSL micro-straws may be useful to store few motile spermatozoa with good recovery of motility for patients undergoing ICSI treatment.