Postprandial plasma bile acid responses in normal weight and obese subjects
Postprandial plasma bile acid responses in normal weight and obese subjects
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DOI：
10.1258/acb.2010.010040
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发表时间：
2010-09-01
影响因子：
2.2
通讯作者：
le Roux, C. W.
中科院分区：
文献类型：
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作者：
Glicksman, C.;Pournaras, D. J.;le Roux, C. W.
背景资料：胆汁酸可以通过各种受体作为信号分子，包括核法尼醇X受体（FXR）和胆甾烷X受体（PXR），以及细胞表面G蛋白偶联受体TGR 5。该信号传导与肽YY（PYY）和胰高血糖素样肽1（GLP-1）的释放有关，这改善了血糖控制和能量消耗。我们调查是否病态肥胖的受试者有改变餐后胆汁酸responses in comparison to normal weight subjects.Method：血液样本，每30分钟从0到180分钟后400千卡的测试餐。样本取自12名体重正常的受试者，体重指数（BMI）为23.2（2.8）kg/m2（中位数[四分位距（IQR）]），7名肥胖患者，BMI为47.2（7.2）kg/m2。分馏胆汁酸测定对这些样品使用高效液相色谱串联mass spectrometry.Results：肥胖受试者表现出较低的餐后反应，总胆汁酸与正常体重的受试者相比。在正常体重和肥胖受试者中分别观察到6.4（5.0）和2.6（3.3）μ mol/L（中位数[IQR]）增加（P = 0.02）。差异主要是由于甘氨酸结合部分（P = 0.03）。有无差异的增加未结合或牛磺酸结合fractions.Conclusions：降低餐后胆汁酸反应肥胖受试者与正常体重受试者相比，可能部分解释了次优GLP-1和PYY反应，并可能影响食欲，血糖控制和能量消耗。
Background: Bile acids can act as signalling molecules via various receptors including the nuclear farnesoid X receptor (FXR) and pregnane X receptor (PXR), and the cell surface G-protein-coupled receptor TGR5. The signalling has been implicated in the release of peptide YY (PYY) and glucagon-like peptide 1 (GLP-1), which improves glycaemic control and energy expenditure. We investigated whether morbidly obese subjects have altered postprandial bile acid responses in comparison to normal weight subjects.Method: Blood samples were taken every 30 min from 0 to 180 min following a 400 kcal test meal. Samples were taken from 12 normal weight subjects with a body mass index (BMI) of 23.2 (2.8) kg/m(2) (median [interquartile range (IQR)]) and seven obese patients with a BMI of 47.2 (7.2) kg/m(2). Fractionated bile acids were measured on these samples using high-performance liquid chromatography tandem mass spectrometry.Results: The obese subjects showed a lower postprandial response in total bile acids compared with the normal weight subjects. An increase of 6.4 (5.0) and 2.6 (3.3) mu mol/L (median [IQR]) in normal weight and obese subjects was observed, respectively (P = 0.02). The difference was predominantly due to the glycine-conjugated fraction (P = 0.03). There was no difference in the increase of the unconjugated or taurine-conjugated fractions.Conclusions: The decreased postprandial bile acid response in obese subjects compared with normal weight subjects may partly explain the suboptimal GLP-1 and PYY responses and could affect appetite, glycaemic control and energy expenditure.