Single-Strand Conformation Polymorphism for Molecular Variability Studies of Six Viroid Species
Single-Strand Conformation Polymorphism for Molecular Variability Studies of Six Viroid Species
复制标题
DOI：
10.1271/bbb.120714
复制
发表时间：
2013-01-01
影响因子：
1.6
通讯作者：
Fakhfakh, Hatem
中科院分区：
文献类型：
--
作者：
Elleuch, Amine;Hamdi, Imen;Fakhfakh, Hatem
首先通过单链构象多态性（SSCP）技术，然后通过直接测序分析，在6个类病毒种和不同的分子变异，在每个物种感染果树的分子多样性估计。研究的不同变异体是3种澳大利亚葡萄藤病毒（AGVd）、4种柑橘矮化类病毒（CDVd）、11种葡萄藤黄色斑点类病毒1型（GYSVd-1）、4种啤酒花矮化类病毒（HSVd）、7种桃潜伏花叶类病毒（PLMVd）和8种梨水泡溃疡类病毒（PBCVd）。对聚丙烯酰胺凝胶电泳（PAGE）条件进行了比较和优化，以提高现有SSCP参数的灵敏度。研究了所观察到的各种SSCP图谱与核苷酸序列变异之间的关系。结果表明，每个物种的电泳的一些参数的变化允许更高的分辨率，因此检测到的单核苷酸变异的克隆最初聚类到同一组。
Molecular diversity within six viroid species and different molecular variants, in each species infecting fruit trees was first estimated by the single-strand conformation polymorphism (SSCP) technique and then by direct sequencing analysis. The different variants studied are to three Australian grapevine viroids(AGVd), four citrus dwarfing viroids (CDVd), eleven grapevine yellow speckle viroids type-1 (GYSVd-1), four hop stunt viroids (HSVd), seven peach latent mosaic viroids (PLMVd), and eight pear blister canker viroids (PBCVd). Polyacrylamide gel electrophoresis (PAGE) conditions were compared and optimized to improve the sensitivity of the existing SSCP parameters. The relationships among the various SSCP profiles observed and the variation in nucleotide sequences was studied. The results indicate that the variations of some parameters of electrophoresis for each species allowed higher resolution and hence detection of single nucleotide variations among clones initially clustered into the same group.