Estradiol induces a shift in cultured cells that release prolactin or growth hormone.
Estradiol induces a shift in cultured cells that release prolactin or growth hormone.
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雌二醇会诱导培养细胞发生变化，释放催乳素或生长激素。
DOI：
10.1152/ajpendo.1986.250.1.e103
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Frawley,LS
中科院分区：
文献类型：
--
作者：
Boockfor,FR;Hoeffler,JP;Frawley,LS
雌二醇诱导大鼠垂体催乳素细胞百分比增加的过程需要澄清。在这项研究中，雌激素治疗的影响，生长激素和催乳素细胞在单层培养的雄性大鼠垂体进行了评估，通过使用反向溶血空斑试验。用17 β-雌二醇（10 nM）处理培养物6天，可显著（P <0.01）提高培养物中释放催乳素的所有细胞的百分比，而不影响生长激素分泌物的百分比。然而，通过连续测定单个细胞的生长激素和催乳素，我们发现，雌二醇引起了显着增加（P <0.01）的比例，个别细胞释放生长激素和催乳素和相应的比例，单独释放生长激素的下降。这些结果表明，雌二醇直接作用于男性垂体细胞的培养物，改变释放生长激素、催乳素或两种激素的细胞的比例，并表明雌二醇可以将仅释放生长激素的细胞转化为同时释放生长激素和催乳素的细胞。
The process by which estradiol induces an increase in the percentage of prolactin cells in the rat pituitary requires clarification. In this study the effect of estradiol treatment on growth hormone and prolactin cells in monolayer cultures of male rat pituitaries was assessed by use of reverse hemolytic plaque assays. Treatment of cultures with 17 beta-estradiol (10 nM) for 6 days elevated significantly (P less than 0.01) the percentage of all cells in culture that released prolactin without affecting the percentage of growth hormone secretors. However, by sequentially assaying single cells for growth hormone and prolactin, we found that estradiol caused a marked increase (P less than 0.01) in the proportion of individual cells that released both growth hormone and prolactin and a commensurate decrease in the proportion that released growth hormone alone. These results demonstrate that estradiol acts directly on cultures of male pituitary cells to shift the proportions of cells that released growth hormone, prolactin, or both hormones and indicate that estradiol may convert cells that release only growth hormone to those that release both growth hormone and prolactin.