The effect of abdominal contrast-enhanced CT on DNA double-strand breaks in peripheral blood lymphocytes: an in vitro and in vivo study
The effect of abdominal contrast-enhanced CT on DNA double-strand breaks in peripheral blood lymphocytes: an in vitro and in vivo study
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DOI：
10.1177/0284185118799513
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发表时间：
2019-06-01
期刊：
影响因子：
1.3
通讯作者：
Zhang, Long Jiang
中科院分区：
文献类型：
--
作者：
Tao, Shu Min;Zhou, Fan;Zhang, Long Jiang
背景由于腹部计算机断层扫描（CT）的辐射剂量可能高于其他器官系统，因此监测这种检查类型的辐射暴露尤为重要。目的探讨腹部CT增强扫描（CE-CT）对外周血淋巴细胞DNA双链断裂（DSB）水平的影响。材料与方法本研究分两部分进行：（i）体外研究：12名志愿者的静脉血样本分为四组。A组中的样品不经历辐射暴露，而B、C和D组分别接受相当于腹部CE-CT扫描的1-3倍辐射剂量的一次CT扫描;和（ii）体内研究：在30名患者中，在CT之前和CT之后5分钟采集血液。分离淋巴细胞并通过γ-H2 AX蛋白的免疫荧光染色。通过方差分析或配对t检验比较DSB水平。使用Pearson相关分析分析辐射剂量与γ-H2 AX病灶增加之间的关系。结果体外实验中，B、C、D组DSB含量分别比A组高49.4%、96.6%、149.4%（均P < 0.001）;四个亚组的放射剂量与DSB水平呈直线相关（P < 0.001）。在体内研究中，CT后DSB水平增加43.5%（P < 0.001）。结论腹部CE-CT在体外和体内实验中均显著增加DSB水平。在体外研究中观察到CT辐射剂量与细胞内DSB水平呈正线性相关。
Background As abdominal computed tomography (CT) radiation dose can be higher compared with other organ systems, monitoring the radiation exposure from this exam type is especially important. Purpose To evaluate the effect of abdominal contrast-enhanced CT (CE-CT) on levels of DNA double-strand breaks (DSBs) in peripheral blood lymphocytes. Material and Methods This study was performed in two parts: (i) an in vitro study: venous blood samples from 12 volunteers were divided into four groups. Samples in group A did not undergo radiation exposure, while groups B, C, and D received one CT scan with 1-3 times the radiation dose equivalent to abdominal CE-CT scan, respectively; and (ii) an in vivo study: blood was taken before CT and 5 min after CT in 30 patients. Lymphocytes were isolated and stained by immunofluorescence of gamma-H2AX protein. DSB levels were compared by variance analysis or paired t-test. The relationship between radiation dose and gamma-H2AX focus increase was analyzed using Pearson correlation analysis. Results In the in vitro study, DSBs levels in groups B, C, and D were 49.4%, 96.6%, and 149.4% higher than those in Group A, respectively (all P < 0.001). Radiation dose in the four subgroups had a linear correlation to DSB levels (P < 0.001). In the in vivo study, the DSB level was 43.5% higher after CT (P < 0.001). Conclusion Abdominal CE-CT significantly increased DSB levels in both in vitro and in vivo experiments. A positive linear correlation of CT radiation dose with intracellular DSBs levels was observed in the in vitro study.