Synthetic strategies for studying embryonic development.
Synthetic strategies for studying embryonic development.
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研究胚胎发育的综合策略。
DOI：
10.1016/j.chembiol.2010.04.013
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发表时间：
2010
影响因子：
--
通讯作者：
Chen,JamesK
中科院分区：
文献类型：
--
作者：
Ouyang,Xiaohu;Chen,JamesK
发育生物学已经从一门描述性科学发展成为一门以遗传学原理和分子机制为基础的科学。虽然分子生物学和基因技术一直是这一转变的主要驱动力，但合成策略已越来越多地被用于以空间和时间的精度询问胚胎模式的机制。在这篇综述中，我们综述了化学工具和工程蛋白质如何被用来在DNA、RNA、蛋白质和细胞水平上干扰发育过程。我们讨论了每种方法的设计原则、实验能力和局限性，以及化学和发育生物学社区未来的挑战。
Developmental biology has evolved from a descriptive science to one based on genetic principles and molecular mechanisms. Although molecular biology and genetic technologies have been the primary drivers of this transformation, synthetic strategies have been increasingly utilized to interrogate the mechanisms of embryonic patterning with spatial and temporal precision. In this review, we survey how chemical tools and engineered proteins have been used to perturb developmental processes at the DNA, RNA, protein, and cellular levels. We discuss the design principles, experimental capabilities, and limitations of each method, as well as future challenges for the chemical and developmental biology communities.