PARKINSONIAN SERUM CARRIES COMPLEMENT-DEPENDENT TOXICITY FOR RAT MESENCEPHALIC DOPAMINERGIC-NEURONS IN CULTURE
PARKINSONIAN SERUM CARRIES COMPLEMENT-DEPENDENT TOXICITY FOR RAT MESENCEPHALIC DOPAMINERGIC-NEURONS IN CULTURE
复制标题
DOI：
10.1016/0006-8993(94)91541-5
复制
发表时间：
1994-01-07
期刊：
影响因子：
2.9
通讯作者：
LEON, A
中科院分区：
文献类型：
--
作者：
DEFAZIO, G;DALTOSO, R;LEON, A
The presence of antibodies recognizing specific epitopes of dopaminergic neurons in serum of patients suffering of Parkinson's Disease (PD) as well as their capability to induce neuronal damage was investigated utilizing serum-free dissociated mesencephalic-striatal co-cultures. High affinity dopamine (DA) and GABA uptakes were assessed as specific, functional markers of dopaminergic and GABAergic cell viability, respectively. Heat-inactivated serum samples from 18 and 13 patients suffering from idiopathic and vascular parkinsonism, respectively and from 18 neurologic controls, were added to co-cultures on day 4 in vitro. Twenty four hours later, reconstituted rabbit complement was added for 60 min and uptake parameters as well as immunocytochemical staining for tyroxyne hydroxylase (TH)-containing cells were subsequently assessed. DA, but not GABA, uptake was significantly decreased only when complement was added to cultures containing serum samples from 14 out of 18 patients with idiopathic parkinsonism and 3 out of 13 patients with vascular parkinsonism (Fisher test, P
