Ultrasensitive cross-correlation electrophoresis on microchip devices
Ultrasensitive cross-correlation electrophoresis on microchip devices
复制标题
微芯片器件上的超灵敏互相关电泳
DOI：
10.1021/ac990853d
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发表时间：
1999
影响因子：
7.4
通讯作者：
J. Michael Ramsey
中科院分区：
文献类型：
--
作者：
J. C. Fister;S. Jacobson;J. Michael Ramsey
Cross-correlation electrophoresis with fluorescence detection on a microchip device was used to resolve a mixture of 63 pM fluorescein and 70 pM dichlorofluorescein. The signal-to-noise ratios of analyte peaks in a correlogram derived from a 9-bit pseudorandom injection sequence were ∼17 for dichlorofluorescein and ∼6 for fluorescein. In contrast, neither peak was detected in a conventional electropherogram obtained on the microchip. Detection limits estimated from a correlogram derived from the average of 12 7-bit correlograms were 6.5 pM dichlorofluorescein and 21 pM fluorescein. These are the lowest detection limits reported for a microchip separation device using analog detection and the first example of cross-correlation electrophoresis on a monolithic device. The small injection volumes, injection reproducibility, and rapid separations enabled by microchip devices make cross-correlation multiplex injection schemes a viable approach for achieving enhanced sensitivity.
