Immunoglobulins drive terminal maturation of splenic dendritic cells
Immunoglobulins drive terminal maturation of splenic dendritic cells
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DOI：
10.1073/pnas.1210654110
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发表时间：
2013-02-05
影响因子：
11.1
通讯作者：
Weiss, Siegfried
中科院分区：
文献类型：
--
作者：
Zietara, Natalia;Lyszkiewicz, Marcin;Weiss, Siegfried
抗原呈递细胞（如树突状细胞DC）的性质和生理状态对于引发的免疫反应是决定性的。已经描述了影响DC成熟的多种因素和细胞相互作用。在这里，我们表明，在体内免疫球蛋白（IG）的情况下产生的DC在可溶性抗原的交叉呈递中受损。这种缺陷是由于抗原对溶酶体的异常细胞靶向及其快速降解。DC的功能可以通过IG的转移而恢复，而与抗原特异性和同种型无关。通过共同应用甘露聚糖抑制IG的交叉呈递的调制，因此，可能是由C型凝集素受体介导的。脾DC对IG交叉呈递抗原的这种意想不到的依赖性提供了对细胞和体液免疫之间的相互作用以及IG的免疫调节能力的深入了解。
Nature and physiological status of antigen-presenting cells, such as dendritic cells DCs, are decisive for the immune reactions elicited. Multiple factors and cell interactions have been described that affect maturation of DCs. Here, we show that DCs arising in the absence of immunoglobulins (Ig) in vivo are impaired in cross-presentation of soluble antigen. This deficiency was due to aberrant cellular targeting of antigen to lysosomes and its rapid degradation. Function of DCs could be restored by transfer of Ig irrespective of antigen specificity and isotype. Modulation of cross-presentation by Ig was inhibited by coapplication of mannan and, thus, likely to be mediated by C-type lectin receptors. This unexpected dependency of splenic DCs on Ig to cross-present antigen provides insights into the interplay between cellular and humoral immunity and the immunomodulatory capacity of Ig.