The production of a macrophage-activating factor from rainbow trout Salmo gairdneri leucocytes.
The production of a macrophage-activating factor from rainbow trout Salmo gairdneri leucocytes.
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从虹鳟鱼 Salmo gairdneri 白细胞中产生巨噬细胞激活因子。
作者：
S. Graham;C. Secombes
虹鲑头、肾和血白细胞在刀豆蛋白A(ConA)刺激后能够分泌一种可溶性巨噬细胞激活因子(MAF)。佛波酯(PMA)作为共刺激剂的存在增加了MAF的产量。含有MAF的培养上清液与驻留或诱导的巨噬细胞孵育48-72小时后，呼吸爆发活性(NBT、NBT、还原和H_2O_2产生)和杀菌活性均增强。加入MAF前的目标培养时间并不影响它们的反应，但为了获得最大的刺激，持续的MAF存在是必要的。
Rainbow trout head kidney and blood leucocytes are shown to be capable of secreting a soluble macrophage-activating factor (MAF) after stimulation with concanavalin A (Con A). The presence of phorbol myristate acetate (PMA) as a co-stimulant increased the production of MAF. Both respiratory burst activity (nitroblue tetrazolium, NBT, reduction and H2O2 production) and bactericidal activity were enhanced after incubation of resident or elicited macrophages with the MAF-containing supernatants for 48-72 hr. The target culture period before the addition of MAF did not affect their responsiveness, but a continuous presence of MAF was necessary for maximal stimulation.